DNA fingerprinting of oil camellia cultivars with SSR markers
DNA fingerprinting of oil camellia cultivars with SSR markers
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SSR标记油茶品种DNA指纹图谱分析
DOI：
10.1007/s11295-015-0966-7
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发表时间：
2016-02-01
影响因子：
2.4
通讯作者：
Yin, Tongming
中科院分区：
文献类型：
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作者：
Chen, Yingnan;Dai, Xiaogang;Yin, Tongming
油茶（Camellia oleiferaAbel.）是一种广泛种植的重要经济植物。在中国，有100多个优良品种被批准用于商业种植，代表了油茶产业的发展基础。由于油茶幼苗经常被交易并被引入新的地方，因此迫切需要开发一种快速可靠的识别工具包，以保护和确保这些资源的有效利用。本研究从山茶花SSR引物库中筛选出10对高度多态性的SSR引物对，并利用这些引物对构建了128个山茶花优良品种的DNA指纹图谱。指纹图谱分析结果表明，除“长林40”和“富阳40”两个品种的DNA指纹图谱完全相似外，其余品种均具有独特的DNA指纹图谱，表明这两个品种可能是同义或营养突变体。在植物新品种的保护和管理中，同义词是一个主要的混淆来源。虽然这两个品种的同义关系需要进一步确认，我们的数据表明，在授权的油茶品种之间的同义词的可能性很大。本研究为油茶优良品种的鉴定和管理以及育种者的知识产权保护提供了理想的遗传工具包。
Oil camellia (Camellia oleiferaAbel.) is a widely cultivated economically important plant. In China, more than 100 elite cultivars have been authorized for commercial plantations, representing the developmental base for the oil camellia industry. Since oil camellia seedlings are frequently traded and introduced to new places, there is an active demand to develop a rapid and reliable identification toolkit to protect and ensure the effective use of these resources. In this study, ten highly polymorphic simple sequence repeat (SSR) primer pairs were screened from the SSR primer database of oil camellia, and DNA fingerprints of 128 elite oil camellia cultivars were constructed with these primer pairs. By analyzing the fingerprinting profiles, all of these cultivars were found to have unique DNA fingerprints, except ‘Changlin 40’ and ‘Fuyang 40’, which shared a complete similarity across all of the fingerprints generated by these ten SSR primer pairs, indicating that they were either synonyms or vegetative mutants. Synonyms are a major source of confusion in protecting and managing new varieties of plants. Although the synonymy of these two cultivars needs to be further confirmed, our data indicated a high possibility of synonyms among the authorized oil camellia cultivars. This study provides a desirable genetic toolkit for the identification and management of the elite oil camellia cultivars, and also for the intellectual property right protection of breeders.