Gene expression changes after seizure preconditioning in the three major hippocampal cell layers
Gene expression changes after seizure preconditioning in the three major hippocampal cell layers
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DOI：
10.1016/j.nbd.2006.12.001
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发表时间：
2007-04-01
影响因子：
6.1
通讯作者：
Dingledine, Raymond
中科院分区：
文献类型：
--
作者：
Borges, Karin;Shaw, Renee;Dingledine, Raymond
啮齿动物癫痫持续状态（SE）后海马的损害主要发生在锥体细胞，而齿状颗粒细胞层（DGCL）除外。在SE前连续2天短暂癫痫发作预处理的大鼠中，海马损伤得到了预防。为了鉴定预处理后改变的神经保护基因和生化途径，我们比较了预处理对通过激光捕获显微镜收获的CA 1和CA 3锥体细胞和DGCL中基因表达的影响。在DGCL中，632个基因的表达发生了改变，而在CA1和CA3锥体细胞层中只有151和58个基因。大多数差异表达的基因调节组织结构和细胞内和细胞外信号传导，包括神经传递。相对于DGCL，在CA1锥体细胞中发生能量代谢转录物的选择性上调。这些结果揭示了广泛的DGCL预处理的转录反应，并提出了几个机制的预处理癫痫发作的神经保护作用。(c)2006年爱思唯尔公司All rights reserved.
Rodents experience hippocampal damage after status epilepticus (SE) mainly in pyramidal cells while sparing the dentate granule cell layer (DGCL). Hippocampal damage was prevented in rats that had been preconditioned by brief seizures on 2 consecutive days before SE. To identify neuroprotective genes and biochemical pathways changed after preconditioning we compared the effect of preconditioning on gene expression in the CA1 and CA3 pyramidal and DGCLs, harvested by laser capture microscopy. In the DGCL the expression of 632 genes was altered, compared to only 151 and 58 genes in CA1 and CA3 pyramidal cell layers. Most of the differentially expressed genes regulate tissue structure and intra- and extracellular signaling, including neurotransmission. A selective upregulation of energy metabolism transcripts occurred in CA1 pyramidal cells relative to the DGCL. These results reveal a broad transcriptional response of the DGCL to preconditioning, and suggest several mechanisms underlying the neuroprotective effect of preconditioning seizures. (c) 2006 Elsevier Inc. All rights reserved.