Screening of folate status with use of dried blood spots on filter paper.
Screening of folate status with use of dried blood spots on filter paper.
复制标题
使用滤纸上的干血斑筛选叶酸状态。
DOI：
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发表时间：
1999
影响因子：
7.1
通讯作者：
Elaine W. Gunter
中科院分区：
文献类型：
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作者：
S. O'broin;Elaine W. Gunter
背景
滤纸上的干血斑 (DBS) 已成为新生儿筛查的一种成功且经济的基质。
目标
我们的目标是开发和评估 DBS 叶酸分析的优化方法并评估 DBS 叶酸稳定性。
设计
通过在含有 0.1%（体积比）Triton X-100 的 5 g 抗坏血酸/L 中超声处理从纸上洗脱 DBS，并根据 DBS 洗脱液叶酸和血红蛋白浓度计算血红蛋白叶酸值（HF；以 pmol/g 表示）。
结果
在标准化超声处理周期中，超过 95% 的 DBS 叶酸被洗脱，并且 DBS 叶酸测定的重现性在运行内（CV：<8%）和运行之间（CV：<9%）都是可接受的。在同时采集的 80 名献血者的血液样本中，指尖穿刺 DBS 和传统静脉方法的 HF 平均值 (+/-1 SD) 分别为 2513 +/- 1144 和 2607 +/- 1195 pmol/g，并且它们相关性良好（r = 0.97，P < 0.001）。 HF 值和红细胞叶酸测量值可以通过使用平均细胞血红蛋白浓度进行相互转换。
结论
DBS 矩阵有潜力成为叶酸筛查研究的廉价且实用的选择。
BACKGROUND
Dried blood spots (DBS) on filter paper have been a successful and economical matrix for neonatal screening.
OBJECTIVE
Our objective was to develop and evaluate an optimized method for DBS folate analysis and to assess DBS folate stability.
DESIGN
DBS were eluted from paper by sonication in 5 g ascorbic acid/L containing 0.1% (by vol) Triton X-100 and hemoglobin folate values (HF; as pmol/g) were calculated from DBS eluate folate and hemoglobin concentrations.
RESULTS
Over 95% of DBS folate was eluted during a standardized sonication cycle and DBS folate assay reproducibility was acceptable both within (CV: <8%) and between (CV: <9%) runs. HF means (+/-1 SD) from finger-stick DBS and conventional venous methods were 2513 +/- 1144 and 2607 +/- 1195 pmol/g, respectively, in blood samples taken concurrently from 80 donors, and they correlated well (r = 0.97, P < 0.001). HF values and erythrocyte folate measures may be interconverted by using the mean cell hemoglobin concentration.
CONCLUSION
The DBS matrix has potential as an inexpensive and practical option for folate screening studies.