Clogging of a limestone fracture by stimulating groundwater microbes.
Clogging of a limestone fracture by stimulating groundwater microbes.
复制标题
通过刺激地下水微生物堵塞石灰岩裂缝。
DOI：
10.1016/s0043-1354(00)00476-0
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发表时间：
2001
期刊：
影响因子：
12.8
通讯作者：
R. Samson
中科院分区：
文献类型：
--
作者：
Nathalie Ross;R. Villemur;Louise Deschênes;R. Samson
生物堵塞在含水层中得到促进，以容纳或修复地下水。在这项研究中，能够检测的水力传导率（K）的微小变化的装置被开发来测量在石灰岩中的单个裂缝的堵塞，以下微生物刺激。骨折断面2.5mm2，长50 cm。在接种石灰石之前，从地下水中分离的16 S rRNA基因的代表性克隆的测序显示出与噬纤维菌属物种的显著联系，弓形杆菌属和根瘤菌属（Rhizobium spp.）已知这些细菌分泌胞外聚合物并形成生物膜。当向接种的石灰石中加入营养物时，8天后K值下降，22天后达到初始值的0.8%（Kfi=340 cm min-1）。这项研究表明，从地下水的土著微生物的刺激有效地堵塞了石灰岩中的宏观裂缝，这表明在裂缝岩石含水层的生物barriers的潜在应用。
Biological clogging is promoted in aquifers either to contain or to remediate groundwater. In this study, an apparatus able to detect small changes in hydraulic conductivity (K) was developed to measure the clogging of a single fracture in limestone, following microbial stimulation. The fracture had a 2.5mm2section and was 50cm long. Prior to the inoculation of the limestone, the sequencing of representative clones from 16S rRNA genes isolated from groundwater, showed significant affiliation with Cytophaga spp., Arcobacter spp. and Rhizobium spp. These bacteria are known to secrete extracellular polymeric substances and form biofilms. When nutrients were added to the inoculated limestone, a decrease in K occurred after 8 days, reaching 0.8% of its initial value after 22 days (Kfi=340 cm min−1). This study showed that a stimulation of indigenous microbes from groundwater effectively clogged a macrofracture in limestone, suggesting the potential application of biobarriers in fractured rock aquifers.