IMMUNOHISTOCHEMICAL STUDIES USING ANTIBODIES TO LEUCINE-ENCEPHALIN - INITIAL OBSERVATIONS ON NERVOUS-SYSTEM OF RAT
IMMUNOHISTOCHEMICAL STUDIES USING ANTIBODIES TO LEUCINE-ENCEPHALIN - INITIAL OBSERVATIONS ON NERVOUS-SYSTEM OF RAT
复制标题
DOI：
10.1016/0306-4522(76)90063-4
复制
发表时间：
1976-01-01
期刊：
影响因子：
3.3
通讯作者：
TERENIUS, L
中科院分区：
文献类型：
--
作者：
ELDE, R;HOKFELT, T;TERENIUS, L
脑啡肽是具有与吗啡相似的药理学性质的肽。用亮氨酸脑啡肽/甲状腺球蛋白结合物免疫豚鼠。免疫荧光组织化学显示，在大鼠神经系统中广泛分布的轴突及其终末系统。脑啡肽样免疫反应的突出网络被发现在一些脑干核和边缘前脑的部分。胃肠道肌间神经丛也含有荧光纤维，免疫荧光阳性分布与脑啡肽的出现相平行。一些已知具有高阿片受体密度的区域也显示出包含脑啡肽样免疫反应性的引人注目的网络。这些发现为脑啡肽包含在靠近阿片受体的神经末梢中的假设提供了形态学支持。
Enkephalins are peptides which have pharmacological properties similar to those of morphine. Guinea pigs were immunized with a leucine-enkephalin/thyroglobulin conjugate. Immunofluorescence histochemistry with antiserum revealed a widely distributed system of axons and their terminals in the nervous system of the rat. Prominent networks of enkephalin-like immunoreactivity were found in some brainstem nuclei and in portions of the limbic forebrain. The myenteric plexus in the gastrointestinal tract also contained fluorescent fibers.The distribution of the positive immunofluorescence parallels the occurrence of enkephalin as revealed by biochemical techniques. Some areas known to have a high opiate receptor density were also shown to contain striking networks of enkephalin-like immunoreactivity. Such findings provide morphological support for the hypothesis that enkephalins are contained in nerve terminals close to opiate receptors.