Expression of estrogen receptor-α and -β mRNA in the brain of Japanese quail embryos
Expression of estrogen receptor-α and -β mRNA in the brain of Japanese quail embryos
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DOI：
10.1002/dneu.20544
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发表时间：
2007-11-01
影响因子：
3
通讯作者：
Halldin, Krister
中科院分区：
文献类型：
--
作者：
Axelsson, Jeanette;Mattsson, Anna;Halldin, Krister
本研究旨在研究雌激素受体（ER）亚型ER α和ER β在日本鹌鹑胚胎脑中的mRNA表达。我们发现ER α和ER β mRNA的表达在6日龄胚胎的整个头部匀浆中，以及在9日龄和12日龄胚胎的脑匀浆中使用实时PCR。在9日龄和12日龄的胚胎中，雌鼠的ER α表达高于雄鼠。我们使用原位杂交检查ER的本地化在第9天和第17天的孵化的男性和女性的大脑部分。第9天，在发育中的内侧视前核（POM）、终纹床核内侧部（BSTm）和下丘脑结节区检测到ER β mRNA。在9日龄胚胎的POM、BSTm和结节区均未检测到ER α信号。在17日龄的胚胎中，ER β在视前区、杏仁带核（TnA）和BSTm中高度表达。ER α mRNA在部分视前区和端脑TnA中表达。在BSTm中没有发现ER α表达，BSTm是一个已知在成人中具有性二态性的区域。在胚胎期，ER β在与性行为相关的大脑区域中的高表达表明ER β在日本鹌鹑大脑的性别分化中起作用。
The present study was conducted to investigate the mRNA expression of the two estrogen receptor (ER) subtypes ER alpha and ER beta in the brain of Japanese quail embryos. We found expression of both ERa and ER beta mRNA in homogenate of whole head from 6-day-old embryos, and in brain homogenate from 9- and 12-dayold embryos using real-time PCR. In 9- and 12-day-old embryos the ER alpha expression was higher in females than in males. We used in situ hybridization to examine the localization of the ERs in sections from male and female brains on day 9 and day 17 of incubation. On day 9, ER beta mRNA was detected in the developing medial preoptic nucleus (POM), in the medial part of the bed nucleus of the striae terminalis (BSTm), and in the tuberal region of the hypo- thalamus. ER alpha signal could not be detected in the POM, the BSTm or the tuberal region in 9-day-old embryos. In 17-day-old embryos, ER beta was highly expressed in the preoptic area, the nucleus Taeniae of the Amygdala (TnA) and the BSTm. Expression of ERa mRNA was detected in parts of the preoptic area and in the telencephalic TnA. No ERa expression was found in the BSTm, an area known to be sexually dimorphic in adults. The high embryonic expression of ER beta in brain areas linked to sexual behavior indicates that ER beta plays a role in sexual differentiation of the Japanese quail brain.