POLYACRYLAMIDE AS AN INFILTRATING AND EMBEDDING MEDIUM FOR VIBRATOME SECTIONING OF HUMAN FETAL CEREBELLUM CONTAINING DII-FILLED AXONS
POLYACRYLAMIDE AS AN INFILTRATING AND EMBEDDING MEDIUM FOR VIBRATOME SECTIONING OF HUMAN FETAL CEREBELLUM CONTAINING DII-FILLED AXONS
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DOI：
10.1016/0165-0270(92)90135-z
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发表时间：
1992-04-01
影响因子：
3
通讯作者：
BIJLANI, V
中科院分区：
文献类型：
--
作者：
HAYARAN, A;BIJLANI, V
聚丙烯酰胺广泛用于电泳分离蛋白质。在这里使用聚丙烯酰胺作为浸润和包埋介质振动切片的人胎儿小脑含有二碘填充轴突的报道。该方法实施简单，具有几个优点：它不需要预先脱水和清除组织;它可以在4 ℃的低温下进行;聚丙烯酰胺既不溶解DiI，也不干扰荧光显微镜下DiI填充曲线的可视化。
Polyacrylamide is widely used for the separation of proteins by electrophoresis. Here the usage of polyacrylamide as an infiltrating and embedding medium for vibratome sectioning of human fetal cerebellum containing DiI-filled axons is reported. The procedure is simple to implement and has several advantages: it does not require prior dehydration and clearing of tissue; it can be carried out at a low temperature of 4-degrees-C; and the polyacrylamide neither dissolves the DiI nor does it interfere with the visualizing of DiI-filled profiles under fluorescence microscope.