Three-dimensional imaging of biological cells with picosecond ultrasonics
Three-dimensional imaging of biological cells with picosecond ultrasonics
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DOI：
10.1063/1.4918275
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发表时间：
2015-04-20
影响因子：
4
通讯作者：
Wright, Oliver B.
中科院分区：
文献类型：
--
作者：
Danworaphong, Sorasak;Tomoda, Motonobu;Wright, Oliver B.
我们使用皮秒超声波成像动物细胞在体外牛主动脉内皮细胞和小鼠脂肪细胞固定到钛涂层蓝宝石。紧密聚焦的超短激光脉冲产生和检测GHz的声脉冲，允许三维成像（x，y和t）的超声波在细胞中的传播与类似的1 μ m的横向和类似的150 nm的深度分辨率。的连续小波变换振幅的光反射率的变化内部和外部的细胞的时间-频率表示显示GHz布里渊振荡，允许的平均声速的细胞和它们的超声波衰减，以获得以及平均体积模量。(C)2015 AIP Publishing LLC.
We use picosecond ultrasonics to image animal cells in vitro-a bovine aortic endothelial cell and a mouse adipose cell-fixed to Ti-coated sapphire. Tightly focused ultrashort laser pulses generate and detect GHz acoustic pulses, allowing three-dimensional imaging (x, y, and t) of the ultrasonic propagation in the cells with similar to 1 mu m lateral and similar to 150 nm depth resolutions. Time-frequency representations of the continuous-wavelet-transform amplitude of the optical reflectivity variations inside and outside the cells show GHz Brillouin oscillations, allowing the average sound velocities of the cells and their ultrasonic attenuation to be obtained as well as the average bulk moduli. (C) 2015 AIP Publishing LLC.