Electrospray mass-spectrometric, spectrophotometric and electrochemical methods do not provide evidence for the binding of nitric oxide by pyocyanine at pH 7
Electrospray mass-spectrometric, spectrophotometric and electrochemical methods do not provide evidence for the binding of nitric oxide by pyocyanine at pH 7
复制标题
DOI：
10.1042/bj3220025
复制
发表时间：
1997-02-15
影响因子：
4.1
通讯作者：
Nakatsu, K
中科院分区：
文献类型：
--
作者：
Vukomanovic, DV;Zoutman, DE;Nakatsu, K
In several recent publications on pyocyanine, its mechanism of action has been attributed to an ability to react with nitric oxide (NO), resulting in the formation of an adduct. We examined the chemical interaction of pyocyanine and NO using electrospray (ES) MS, spectrophotometry and voltammetry at neutral pH and with 10-100 mu M pyocyanine. No binding of NO to pyocyanine was observed. Alternative mechanisms for the inhibition of NO-induced vasorelaxation of pyocyanine should be sought.
