Proteomic Analysis of Desiccation Tolerance and Its Re-Establishment in Different Embryo Axis Tissues of Germinated Pea Seeds
Proteomic Analysis of Desiccation Tolerance and Its Re-Establishment in Different Embryo Axis Tissues of Germinated Pea Seeds
复制标题
发芽豌豆种子不同胚轴组织耐干燥性及其重建的蛋白质组学分析
DOI：
10.1021/acs.jproteome.0c00860
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发表时间：
2021
影响因子：
4.4
通讯作者：
Song Song-Quan
中科院分区：
文献类型：
--
作者：
Wang Wei-Qing;Wang Yue;Song Xian-Jun;Zhang Qi;Cheng Hong-Yan;Liu Jun;Song Song-Quan
萌发种子脱水耐性（DT）的丧失和重建模型已被广泛用于探讨DT的相关机制，但该模型中的组织变异研究较少。在此，我们研究了DT在萌发的豌豆种子的不同胚轴组织和它的重建聚（乙二醇）（PEG）处理，然后采用iTRAQ为基础的蛋白质组学方法来探讨潜在的机制。萌发种子胚轴各部分的DT变化规律为：上胚轴>下胚轴E（下胚轴部分附着于上胚轴）>下胚轴R（下胚轴部分附着于胚根）>胚根。同时，PEG处理发芽种子导致DT重建在这些组织中的差异程度。萌发种子的下胚轴和上胚轴中分别富集了参与解毒和胁迫反应的蛋白质。复水后，蛋白质组变化在脱水过程中恢复在下胚轴-E，但不是在胚根。PEG处理的发芽种子导致大量的蛋白质的变化，在丰富的干燥敏感的胚根和下胚轴-R，其中许多积累在下胚轴-E和上胚轴处理前。我们推测，第1和第5组莱亚蛋白质和蛋白质的积累相关的解毒，阿坝，乙烯和钙信号转导的DT在不同的组织和它的重建的变化主要贡献。
The model of loss and re-establishment of desiccation tolerance (DT) in germinated seeds has been well developed to explore the mechanisms associated with DT, but little attention has been paid to the tissue variation in this model. Herein, we investigated DT in different embryo axis tissues of germinated pea seeds and its re-establishment by poly(ethylene glycol) (PEG) treatment and then employed an iTRAQ-based proteomic method to explore the underlying mechanisms. DT varied among the four embryo axis parts of germinated seeds: epicotyl > hypocotyl-E (hypocotyl part attached to the epicotyl) > hypocotyl-R (hypocotyl part attached to the radicle) > radicle. Meanwhile, PEG treatment of germinated seeds resulted in a differential extent of DT re-establishment in these tissues. Proteins involved in detoxification and stress response were enriched in desiccation-tolerant hypocotyls-E and epicotyls of germinated seeds, respectively. Upon rehydration, proteome change during dehydration was recovered in the hypocotyls-E but not in the radicles. PEG treatment of germinated seeds led to numerous changes in proteins, in abundance in desiccation-sensitive radicles and hypocotyls-R, of which many accumulated in the hypocotyls-E and epicotyls before the treatment. We hypothesized that accumulation of groups 1 and 5 LEA proteins and proteins related to detoxification, ABA, ethylene, and calcium signaling contributed mainly to the variation of DT in different tissues and its re-establishment.