Changes in cell wall polysaccharides during elongation in a 2, 4‐D free medium in a carrot suspension culture
Changes in cell wall polysaccharides during elongation in a 2, 4‐D free medium in a carrot suspension culture
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胡萝卜悬浮培养物中 2、4-D 游离培养基伸长过程中细胞壁多糖的变化
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发表时间：
1984
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通讯作者：
A. Komamine
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作者：
H. Masuda;Y. Ozeki;S. Amino;A. Komamine
胡萝卜(Daucus carota L.EV.Kurodagosun)细胞通过筛分继代培养(Y.Ozeki和A.Komamin，Physiol.种。53：570-577。1981)转移到缺乏生长素的培养基中，而在含有2，4-D的培养基中细胞没有伸长。研究了细胞壁多糖及其糖组成在伸长过程中的变化。所有的壁组分、EDTA可溶性果胶物质、5%和24%的EDTA可溶性果胶物质、KOH可溶性半纤维素和纤维素在伸长过程中显著增加。半纤维素含量的增加与伸长率尤其相关。在5%KOH可溶半纤维素中，半乳糖和阿拉伯糖的含量在伸长过程中显著增加(单位鲜重)和相对含量(占中性糖总量的%)，而葡萄糖和木糖的相对含量在5%和24%KOH可溶半纤维素中迅速下降。甲基化分析初步表明，与非伸长细胞相比，伸长细胞的两种半纤维素中半乳糖和/或阿拉伯半乳糖的含量较高，而木聚糖的含量较低。伸长培养中胞外多糖的总碳水化合物和糖醛酸的分泌量比非伸长培养中的增加幅度更大。在伸长培养过程中，胞外多糖中半乳糖和阿拉伯糖的含量迅速增加。根据所获得的结果，讨论了在没有生长素的情况下细胞伸长的生化方面。
Cell elongation occurred when carrot (Daucus carota L. ev. Kurodagosun) cells subcultured through sieving (Y. Ozeki and A. Komamine, Physiol. Plant. 53: 570-577. 1981) were transferred to a medium lacking auxin, while the cells showed no elongation in a medium containing 2, 4-D. Changes in polysaccharides of the cell walls and in their sugar composition during elongation were investigated. All wall components, EDTA-soluble pectic substance, 5 and 24%, KOH-soluble hemicelluloses and cellulose increased markedly during elongation. The increase of hemicelluloses correlated especially with elongation. In the 5% KOH-soluble hemicellulose, galactose and arabinose contents in the walls increased significantly both in amounts (per fresh weight) and relative contents (% in total neutral sugars) during elongation, while the relative contents of glucose and xylose decreased rapidly in the 5 and 24% KOH-soluble hemicelluloses. The methylation analysis tentatively indicated that larger amounts of galactan and/or arabinogalactan and lower amount of xyloglucan were found as components of the two hemicelluloses of elongating cells than those of non-elongating cells. The amounts of total carbohydrate and of uronic acid of extracellular polysaccharides secreted into the medium increased to a larger extent in the elongation culture than in the non-elongation culture. The contents of galactose and arabinose in extracellular polysaccharides increased rapidly in the elongation culture. The biochemical aspects of cell elongation in the absence of auxin were discussed from the viewpoint of the results obtained here.