Elevation of interleukin-10 levels in malignant pleural effusion
Elevation of interleukin-10 levels in malignant pleural effusion
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DOI：
10.1378/chest.110.2.433
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发表时间：
1996-08-01
期刊：
影响因子：
9.6
通讯作者：
Perng, RP
中科院分区：
文献类型：
--
作者：
Chen, YM;Yang, WK;Perng, RP
研究目的：湖南免疫已发现有细胞免疫和体液免疫两大组成部分。 1 型辅助 T (Th1) 途径有利于细胞免疫，Th2 途径有利于体液免疫。免疫反应中 Th1 和 Th2 细胞的早期确定取决于白细胞介素 12 (IL-12)（有利于 Th1 反应）和 IL-4（有利于 Th2 反应）之间的平衡。 IL-2 和干扰素-γ (IFN-γ) 在 Th1 途径中产生，IL-4 和 IL-10 在 Th2 途径中产生。据报道，恶性胸腔积液中缺乏细胞免疫、IL-2 和 IFN-γ。然而，据我们所知，此前还没有涉及Th1或Th2途径的其他细胞因子成分的报道。本研究旨在回答这些问题。设计：采用酶联免疫吸附法分析21例恶性胸腔积液患者外周血和胸水的细胞因子水平，包括IL-4、IL-10和IL-12。还采用流式细胞术对外周血和胸腔积液的淋巴细胞亚群进行了研究。测量和结果：结果显示与血液样本相比，IL-10水平显着升高。血液和积液中的 IL-4 和 IL-12 均低于最低可检测浓度。胸膜辅助 T 细胞的比例显着高于血液中的比例 (p=0.0002)。胸膜自然杀伤 (NK) 细胞的比例显着低于血液中的比例 (p=0.0001)。胸膜抑制性 T 细胞的比例低于血液，具有临界意义 (p=0.0522)。血液和胸腔积液之间的 B 淋巴细胞比率未发现显着变化 (p=0.2471)。胸腔积液和血液标本中IL-10水平与淋巴细胞亚群差异无相关性。结论：恶性胸腔积液中辅助性T细胞亚群增多，NK细胞和抑制性T细胞亚群减少。肿瘤性胸腔积液中 NK 细胞亚群减少，IL-10 升高且 IL-12 浓度最低，提示使用 IL-12 或 IL-10 抗体来改善局部细胞免疫。还需要进一步研究。
Study objective: Hunan immunity has been found to have two major components, cellular and humoral immunity. T-helper type 1 (Th1) pathway favors cellular immunity and Th2 pathway favors humoral immunity. Early determination toward Th1 and Th2 cells in the immune response is dependent on the balance between interleukin-12 (IL-12), which favors Th1 responses, and IL-4, which favors Th2 responses. IL-2 and interferon-gamma (IFN-gamma) are produced in the Th1 pathway, and IL-4 and IL-10 are produced in the Th2 pathway. Lack of cellular immunity, IL-2, and IFN-gamma had been reported in malignant pleural effusions. However, to our knowledge, there are no previous reports on other cytokine components involving Th1 or Th2 pathway. The present study was designed to answer these questions.Design: Cytokine levels in peripheral blood and pleural fluid of 21 patients with malignant pleural effusion, including IL-4, IL-10, and IL-12, were analyzed with enzyme-linked immunosorbent assays. Lymphocyte subpopulations of peripheral blood and pleural effusion were also studied by using flow cytometry.Measurements and results: The results showed a significant increase in IL-10 level as compared with blood samples. IL-4 and IL-12 were below minimal detectable concentrations both in the blood and the effusion. The ratio of pleural helper T cells was significantly higher than in the blood (p=0.0002). The ratio of pleural natural killer (NK) cells was significantly lower than in the blood (p=0.0001). The ratio of pleural suppressor T cells was lower than blood with borderline significance (p=0.0522). No significant change in B-lymphocyte ratio between blood and pleural effusion was found (p=0.2471). There was no correlation between difference in IL-10 level and lymphocyte subpopulation of pleural effusion and blood samples.Conclusions: Helper T-cell subpopulations were increased while NK and suppressor T-cell subpopulations were decreased in malignant pleural effusions. The decrease in NK cell subpopulations with elevated IL-10 and minimal IL-12 concentration in neoplastic pleural effusion would suggest the usage of IL-12 or antibody of IL-10 to improve local cellular immunity. Further study is needed.