Identification and comparative analysis of microRNAs in barnyardgrass (Echinochloa crus-galli) in response to rice allelopathy
Identification and comparative analysis of microRNAs in barnyardgrass (Echinochloa crus-galli) in response to rice allelopathy
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DOI：
10.1111/pce.12492
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发表时间：
2015-07-01
影响因子：
7.3
通讯作者：
Lin, Wenxiong
中科院分区：
文献类型：
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作者：
Fang, Changxun;Li, Yingzhe;Lin, Wenxiong
水稻化感作用是化感作用研究领域的一个热点问题，对供体化感水稻的化感行为已有大量的研究。然而，很少有研究涉及受体稗草（BYG）的反应。结果表明，与化感水稻品种PI312777共培养的BYG植株中，与植物激素信号转导、核苷酸切除修复、过氧化物酶体增殖物激活受体和p53信号通路相关的miRNAs表达增强，这些miRNAs在BYG植株中的表达水平与共培养水稻品种的化感潜力呈正相关。用水稻产生的酚酸处理BYG植物也增加了BYG中的miRNA表达，而用水稻产生的萜类化合物处理对miRNA表达没有明显影响。在水培系统中，最大数量的粘球菌被发现在生长培养基中含有最高的水稻化感潜力。在水培培养基中加入酚酸也增加了粘球菌的数量。更有趣的是，接种黄色粘球菌显著增加了处理过的BYG中的miRNA表达。阿魏酸和黄花茅联合处理对BYG的生长抑制作用最强。本研究发现，化感水稻释放的酚类物质介导了根际粘球菌种群的形成，从而影响了稗草中miRNAs的表达，从而抑制了稗草的生长。这是首次确定化感作用靶向受体植物的分子反应，表明化感作用供体和受体植物之间存在复杂的相互作用。
Rice allelopathy is a hot topic in the field of allelopathy, and behaviour of donor allelopathic rice has been well documented. However, few study addresses response of receiver barnyardgrass (BYG). We found that expression of miRNAs relevant to plant hormone signal transduction, nucleotide excision repair and the peroxisome proliferator-activated receptor and p53 signalling pathways was enhanced in BYG co-cultured with the allelopathic rice cultivar PI312777, the expression levels of these miRNAs in BYG plants were positively correlated with allelopathic potential of the co-cultured rice varieties. Treatment of BYG plants with rice-produced phenolic acids also increased miRNA expression in BYG, while treatment with rice-produced terpenoids had no obvious effect on miRNA expression. In the hydroponic system, the largest number of Myxococcus sp. was found in the growth medium containing rice with the highest allelopathic potential. The addition of phenolic acids in the hydroponic medium also increased the number of Myxococcus sp. More interestingly, inoculation with Myxococcus xanthus significantly increased miRNA expression in the treated BYG. Jointed treatments of ferulic acid and M.xanthus led to strongest growth inhibition of BYG. The results suggest that there exist involvement of Myxococcus sp. and mediation of miRNA expression in rice allelopathy against BYG.This study found that phenolic compounds released from allelopathic rice mediated populations of Myxococcus sp. in the rhizosphere, thereby influenced miRNAs expression in barnyardgrass, which led to growth inhibition of the weed. This is the first time to determine molecular response of allelopathy targeted receiver plant, suggesting complicate interactions between allelopathy donor and receiver plants.