Degradation of human hemoglobin by Prevotella intermedia
Degradation of human hemoglobin by Prevotella intermedia
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DOI：
10.1016/j.anaerobe.2006.09.001
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发表时间：
2006-10-01
期刊：
影响因子：
2.3
通讯作者：
Wu, Jun-Zheng
中科院分区：
文献类型：
--
作者：
Guan, Su-Min;Nagata, Hideki;Wu, Jun-Zheng
在这项研究中，中间普雷沃氏菌，专性厌氧杆，降解人血红蛋白的能力进行了测定，通过SDS-PAGE和扫描光密度定量的降解。细菌细胞和培养上清液都降解血红蛋白。中间毕赤酵母对血红蛋白的降解具有时间依赖性、温度敏感性和pH相关性，不受铁限制的影响。抑制研究表明，半胱氨酸蛋白酶可能参与血红蛋白的降解，这种蛋白酶可能需要金属离子的活性，它可能是硫醇需要和胰蛋白酶诱导。结果表明，中间藻能够从血红蛋白中释放血红素，从而为其增殖提供铁源。(c)2006爱思唯尔有限公司版权所有。
In this study, the ability of Prevotella intermedia, an obligate anaerobic rod, to degrade human hemoglobin was determined by SDS-PAGE and the degradation was quantified by scanning densitometry. Both bacterial cells and culture supernatants degraded hemoglobin. The hemoglobin degradation by P. intermedia was time-dependent, heat sensitive, pH related and was not influenced by iron restriction. Inhibition studies demonstrated that a cysteine protease might be involved in hemoglobin degradation and this protease might require metal ions for its activity and it might be thiol-requiring and trypsin-inducible. The results indicate that P. intermedia is capable to release heme from hemoglobin, hence provide a source of iron for its proliferation. (c) 2006 Elsevier Ltd. All rights reserved.