Overexpression of tobacco ethylene response factor NtERF3 gene and its homologues from tobacco and rice induces hypersensitive response-like cell death in tobacco
Overexpression of tobacco ethylene response factor NtERF3 gene and its homologues from tobacco and rice induces hypersensitive response-like cell death in tobacco
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DOI：
10.1007/s10327-011-0355-5
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发表时间：
2012-01-01
影响因子：
1.2
通讯作者：
Matsushita, Yasuhiko
中科院分区：
文献类型：
--
作者：
Ogata, Takuya;Kida, Yuma;Matsushita, Yasuhiko
在植物特异性转录因子中，乙烯反应因子（ERFs）是最大的家族之一。ERFs是植物界所特有的，被认为在植物对各种生物和非生物环境胁迫的响应中起着至关重要的作用。在这里，我们报告的功能分析的转录抑制因子，NtERF3，与细胞死亡相关的过敏反应（HR），植物特异性的抗病原体反应。在烟草花叶病毒（TMV）感染诱导HR过程中，NtERF3的表达上调。通过农杆菌介导的基因递送瞬时过表达NtERF 3可诱导烟草HR样细胞死亡，这与活性氧簇的产生和离子泄漏有关。从NtERF3中删除ERF相关的两亲性阻遏（ERF）基序不会诱导细胞死亡，而删除对蛋白质的核定位没有影响。在病毒介导的基因传递后，在没有N基因的烟草中也观察到类似的结果。除了NtERF3，其他含有ERFs的烟草，本塞姆氏烟草和水稻ERFs也诱导细胞死亡时，在烟草植物过量生产。这些结果表明，许多ERF基因编码的含ERF基序的蛋白可能具有诱导细胞死亡的能力时，过表达。
Among plant-specific transcription factors, ethylene response factors (ERFs) comprise one of the largest families. ERFs are unique to the plant kingdom and are considered to have crucial roles in plant response to various biotic and abiotic environmental stresses. Here, we report on the functional analysis of a transcriptional repressor, NtERF3, with regard to cell death associated with a hypersensitive response (HR), a plant-specific resistance reaction against pathogens. Expression of NtERF3 was upregulated during HR induction by Tobacco mosaic virus (TMV) infection in tobacco plants harboring the resistance N gene to TMV. Transient overexpression of NtERF3 by Agrobacterium-mediated gene delivery induced HR-like cell death in tobacco, associated with the production of reactive oxygen species and ion leakage. Deletion of the ERF-associated amphiphilic repression (EAR) motif from NtERF3 resulted in no induction of cell death, while the deletion had no effect on nuclear localization of the proteins. After virus-mediated gene delivery, similar results also were observed in tobacco without the N gene. In addition to NtERF3, other EAR motif-containing ERFs from tobacco, Nicotiana benthamiana and rice also induced cell death when overproduced in tobacco plants. The results suggested that many ERF genes encoding EAR motif-containing proteins might have the ability to induce cell death when overexpressed.