Development of biotin molecule targeted cancer cell drug delivery of doxorubicin loaded κ-carrageenan grafted graphene oxide nanocarrier
Development of biotin molecule targeted cancer cell drug delivery of doxorubicin loaded κ-carrageenan grafted graphene oxide nanocarrier
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DOI：
10.1016/j.msec.2019.03.011
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发表时间：
2019-07-01
影响因子：
7.9
通讯作者：
Rajan, Mariappan
中科院分区：
文献类型：
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作者：
Vinothini, Kandasamy;Rajendran, Naresh Kumar;Rajan, Mariappan
宫颈癌是世界上发病率最高的癌症之一，也是女性癌症的第四大发病率。在这项研究中，我们计划合成Kappa-Carrageenan接枝石墨烯氧化物纳米载体与生物素(GO-Kappa-Car-Biotin)，用于靶向宫颈癌。阿霉素(DOX)是一种广为人知的抗癌药物，用于通过pi-pi堆积作用将其包裹在氧化石墨烯表面。用傅立叶变换红外光谱(FT-IR)和X射线衍射仪(XRD)对合成的纳米载体的化学功能和结晶性质进行了表征。用扫描电子显微镜、透射电子显微镜和原子力显微镜对其表面形貌进行了研究。用紫外-可见光谱仪测定了DOX在最大吸收波长下的体外释药曲线。值为480 nm。DOX在GO-kappa-CAR-生物素上的包封率为94%。该亲水性DOX药物在体外研究中具有极好的pH敏感性药物释放。用HeLa细胞株体外检测了合成的GO基纳米载体的抗癌活性。采用台盼蓝比色法、三磷酸腺苷(ATP)比色法、乳酸脱氢酶(LDH)比色法和Hoechst染色分别检测细胞存活率、增殖活性、细胞毒性和核染色质凝集。最后，生物素领先的Go-kappa-Car载体被证明是一种很有前途的宫颈癌治疗药物输送系统。
Cervical cancer is one of the most occurring cancers and the fourth leading occurrence of cancer in women, worldwide. In this study, we planned to synthesis kappa-Carrageenan grafted graphene oxide nanocarrier conjugated with biotin (GO-kappa-Car-biotin) for targeted cervical cancer. Doxorubicin (DOX) is a well-known anticancer drug for any type of cancer and it is used to entrap over on the graphene oxide surface via pi-pi stacking interaction. The chemical function and crystalline nature of the synthesized nanocarrier was characterized by Fourier Transformed Infrared Spectroscopy (FT-IR) and X-ray diffraction Analysis (XRD). The surface morphological study was carried out through Scanning Electron Microscopy (SEM), Transmission electron microscopy (TEM) and Atomic force microscopy (AFM). The in-vitro drug release profile of DOX was carried out by UV-Vis spectrometer at the lambda(max). value of 480 nm. The entrapment of DOX on GO-kappa-car-biotin has been observed at 94%. The hydrophilic DOX drug has excellent pH-sensitive drug released in an in-vitro study. The anticancer efficiency of the synthesized GO-based nanocarrier was examined using HeLa cell line in-vitro. Cell viability, proliferation, cytotoxicity, and nuclear chromatin condensation was studied by trypan blue assay, triphosphate assay (ATP), lactate dehydrogenase assay (LDH) and Hoechst staining respectively. Finally, biotin leading GO-kappa-Car carrier demonstrated is a promising drug delivery system for cervical cancer treatment.