Close contacts between sigma 37-RNA polymerase and a Bacillus subtilis chromosomal promoter.
Close contacts between sigma 37-RNA polymerase and a Bacillus subtilis chromosomal promoter.
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sigma 37-RNA 聚合酶与枯草芽孢杆菌染色体启动子之间密切接触。
DOI：
10.1016/0022-2836(82)90399-0
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发表时间：
1982
影响因子：
5.6
通讯作者：
Losick,R
中科院分区：
文献类型：
--
作者：
MoranJr,CP;Johnson,WC;Losick,R
我们已经鉴定了一种新型枯草芽孢杆菌RNA聚合酶（含有37，000分子量的σ因子（σ37））和同源染色体启动子spoVC之间的密切联系。密切接触者通过检查嘌呤甲基化的模式由硫酸二甲酯随后通过RNA聚合酶结合可视化。鉴定了5种鸟嘌呤和4种腺嘌呤，它们的甲基化被含σ37的RNA聚合酶增强或抑制。这些碱基聚集在两个区域（集中在转录起始点之前的35和10个碱基对），这两个区域对应于已知在B中RNA聚合酶全酶的主要形式识别启动子中重要的区域。枯草杆菌（含σ55）和大肠杆菌。
We have identified close contacts between a novel form ofBacillus subtilisRNA polymerase containing a 37,000 molecular weight species of sigma factor (σ37) and a cognate chromosomal promoterspoVC. Close contacts were visualized by examining the pattern of purine methylation by dimethyl sulphate subsequent to binding by RNA polymerase. Five guanines and four adenines were identified whose methylation was enhanced or suppressed byσ37-containing RNA polymerase. These bases were clustered in two regions (centered at 35 and 10 base-pairs preceding the start-point of transcription) that corresponded inpositionto regions known to be important in promoter recognition by the principal forms of RNA polymerase holoenzyme inB. subtilis(containingσ55) andEscherichia coli.