Differences in protein expression among five species of stream stonefly (Plecoptera) along a latitudinal gradient in Japan
Differences in protein expression among five species of stream stonefly (Plecoptera) along a latitudinal gradient in Japan
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DOI：
10.1002/arch.21422
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发表时间：
2017-11-01
影响因子：
2.2
通讯作者：
Watanabe, Kozo
中科院分区：
文献类型：
--
作者：
Gamboa, Maribet;Tsuchiya, Maria Claret;Watanabe, Kozo
蛋白质组变化之间的自然种群沿着的环境梯度，可以提供洞察物种的生物功能是如何与他们的地方适应。我们研究了五个流石松物种的蛋白质表达，从四个地理区域沿着纬度梯度在日本不同的气候条件。提取的蛋白质通过二维凝胶电泳进行分离，并通过基质辅助激光解吸/电离飞行时间（MALDI TOF/TOF）进行鉴定，得到446个蛋白质。在地理区域内的5个物种之间观察到低种间差异的蛋白质组谱，大概是由于共同发生的物种共享的环境。然而，大的空间差异蛋白质表达被发现在四个地理区域之间，这表明在异质性环境中的蛋白质表达的强烈调节，其中的空间变化与水温呈正相关。我们鉴定了21种在一个地理区域特异性表达的独特蛋白质和6种在所有区域表达的常见蛋白质。在较温暖的地区，代谢蛋白上调，而与冷应激、光周期和交配相关的蛋白下调。在海拔较高的寒冷地区，氧相关蛋白和能量生产蛋白上调。因此，我们的蛋白质组学方法是有用的识别和理解相关的石蝇种群的本地适应的重要生物功能。
Proteome variation among natural populations along an environmental gradient may provide insights into how the biological functions of species are related to their local adaptation. We investigated protein expression in five stream stonefly species from four geographic regions along a latitudinal gradient in Japan with varying climatic conditions. The extracted proteins were separated by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption/ionization of time-of-flight (MALDI TOF/TOF), yielding 446 proteins. Low interspecies variation in the proteome profiles was observed among five species within geographical regions, presumably due to the co-occurring species sharing the environments. However, large spatial variations in protein expression were found among four geographic regions, suggesting strong regulation of protein expression in heterogeneous environments, where the spatial variations were positively correlated with water temperature. We identified 21 unique proteins expressed specifically in a geographical region and six common proteins expressed throughout all regions. In warmer regions, metabolic proteins were upregulated, whereas proteins related to cold stress, the photoperiod, and mating were downregulated. Oxygen-related and energy-production proteins were upregulated in colder regions with higher altitudes. Thus, our proteomic approach is useful for identifying and understanding important biological functions related to local adaptations by populations of stoneflies.