Basal forebrain cholinergic neurons are necessary for estrogen to enhance acquisition of a delayed matching-to-position T-maze task
Basal forebrain cholinergic neurons are necessary for estrogen to enhance acquisition of a delayed matching-to-position T-maze task
复制标题
DOI：
10.1006/hbeh.2002.1825
复制
发表时间：
2002-11-01
影响因子：
3.5
通讯作者：
Gibbs, RB
中科院分区：
文献类型：
--
作者：
Gibbs, RB
间隔内注射选择性胆碱能免疫毒素 192 IgG-皂素 (SAP)，以确定基底前脑胆碱能神经元对于延迟匹配位置 (DMP) T 迷宫任务中激素介导的获取增强是否是必需的。 DMP任务是一个简单的空间学习任务。研究表明，连续雌二醇替代可以增强年轻卵巢切除大鼠 DMP 任务的获得，而长期使用雌二醇或雌二醇 + 黄体酮治疗可以防止老年大鼠 DMP 获得的缺陷。在本研究中，连续雌二醇替代显着增强了未接受 SAP 治疗的卵巢切除大鼠完成 DMP 任务的能力。相比之下，连续雌二醇或每周施用雌二醇+黄体酮均未显着增强接受间隔内注射高剂量（1.0杯）或低剂量（0.22杯）SAP的大鼠的DMP任务获得。无论进行何种治疗，达到标准的动物都会因将迷宫旋转 180 度而受到显着损害，这表明所有组中的动物至少在某种程度上使用迷宫外线索来解决任务。与未接受 SAP 治疗的对照组相比，接受 SAP 治疗的动物对增加间期延迟稍微敏感一些，这表明 SAP 损伤会造成空间工作记忆的适度缺陷。免疫组织化学证实 SAP 治疗动物基底前脑特定区域的胆碱能神经元丢失。此外，DMP 的获取与海马和额叶皮层的 ChAT 活性显着相关。数据表明，基础前脑胆碱能投射对于激素介导的 DMP 获取增强是必要的。 (C) 2002 年爱思唯尔科学（美国）。
Intraseptal injections of the selective cholinergic immunotoxin 192 IgG-saporin (SAP) were performed to determine whether basal forebrain cholinergic neurons are necessary for hormone-mediated enhancement of acquisition in a delayed matching-to-position (DMP) T-maze task. The DMP task is a simple spatial learning task. Studies have shown that continuous estradiol replacement enhances acquisition of the DMP task in young ovariectomized rats and that long-term treatment with either estradiol or estradiol + progesterone can prevent a deficit in DMP acquisition in old rats. In the present study, continuous estradiol replacement significantly enhanced acquisition of the DMP task by non-SAP-treated, ovariectomized rats. In contrast, neither continuous estradiol nor weekly administration of estradiol + progesterone significantly enhanced acquisition of the DMP task in rats that received intraseptal injections of either a high dose (1.0 mug) or a low dose (0.22 mug) of SAP. Animals that reached criterion were significantly impaired by rotating the maze 180 regardless of treatment, suggesting that animals in all groups used extramaze cues to at least some degree to solve the task. SAP-treated animals were slightly more sensitive to increasing the intertrial delay than non-SAP-treated controls, suggesting that the SAP lesions produced a modest deficit in spatial working memory. Immunohistochemistry confirmed the loss of cholinergic neurons in specific regions of the basal forebrain of SAP-treated animals. In addition, DMP acquisition correlated significantly with ChAT activity in the hippocampus and frontal cortex. The data suggest that basal forebrain cholinergic projections are necessary for hormone-mediated enhancement of DMP acquisition. (C) 2002 Elsevier Science (USA).