Genome-wide identification of calcium-dependent protein kinases in soybean and analyses of their transcriptional responses to insect herbivory and drought stress.
Genome-wide identification of calcium-dependent protein kinases in soybean and analyses of their transcriptional responses to insect herbivory and drought stress.
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大豆钙依赖性蛋白激酶的全基因组鉴定及其对昆虫食草和干旱胁迫的转录反应分析
DOI：
10.1038/srep18973
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发表时间：
2016-01-06
影响因子：
4.6
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Hettenhausen C;Sun G;He Y;Zhuang H;Sun T;Qi J;Wu J
钙依赖性蛋白激酶（CDPKs）是植物特有的钙离子传感器，在植物生理的各个方面发挥着重要作用。在这里，我们研究了大豆（Glycine max）GmCDPKs的遗传关系，染色体位置，基因结构和组织特异性，植食性和干旱诱导的表达谱。共鉴定出50个GmCDPK基因，它们在遗传学上分为4个不同的簇，分布在13个亚簇中。GmCDPKs的mRNA剪接位点高度保守，其外显子数目和长度与系统发育关系一致，表明在单子叶植物和真双子叶植物分化之前，至少有13个祖先CDPKs基因已经出现。基因表达分析表明，GmCDPKs在植物组织中具有特异性表达，其转录水平在创伤后发生变化，在模拟甜菜夜蛾取食或大豆蚜取食后表现出特异性表达，且在很大程度上不依赖于植物激素茉莉酸和水杨酸。在干旱和脱落酸处理后，检测到最明显的转录反应，超过一半的GmCDPKs被上调，这表明它们在大豆非生物胁迫响应中的重要作用。我们的数据提供了一个重要的基础，进一步的功能解剖GmCDPKs，特别是在大豆-昆虫相互作用和干旱胁迫适应的背景下。
Calcium-dependent protein kinases (CDPKs) are plant-specific calcium sensors that play important roles in various aspects of plant physiology. Here, we investigated phylogenic relationships, chromosomal locations, gene structures and tissue-specific, herbivory- and drought-induced expression profiles of soybean (Glycine max)GmCDPKs. FiftyGmCDPKgenes were identified, which phylogenetically grouped into 4 distinct clusters and distributed across 13 sub-clusters. Individual classes ofGmCDPKs harbor highly conserved mRNA splicing sites and their exon numbers and lengths were consistent with the phylogenetic relationships, suggesting that at least 13 ancestralCDPKgenes had emerged before the split of monocots and eudicots. Gene expression analysis indicated that severalGmCDPKs were tissue-specific expressed.GmCDPKs’transcript levels changed after wounding, exhibited specific expression patterns after simulatedSpodoptera exiguafeeding or soybean aphid (Aphis glycines) herbivory and were largely independent of the phytohormones jasmonic acid and salicylic acid. The most pronounced transcriptional responses were detected after drought and abscisic acid treatments with more than half of allGmCDPKsbeing upregulated, suggesting their important roles during abiotic stress responses in soybean. Our data provide an important foundation for further functional dissection of GmCDPKs, especially in the context of soybean-insect interactions and drought stress adaptation.