Development of a semiquantitative degenerate real-time PCR-based assay for estimation of numbers of butyryl-coenzyme A (CoA) CoA transferase genes in complex bacterial samples
Development of a semiquantitative degenerate real-time PCR-based assay for estimation of numbers of butyryl-coenzyme A (CoA) CoA transferase genes in complex bacterial samples
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DOI：
10.1128/aem.02561-06
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发表时间：
2007-03-01
影响因子：
4.4
通讯作者：
Flint, Harry J.
中科院分区：
文献类型：
--
作者：
Louis, Petra;Flint, Harry J.
我们描述了一种基于检测丁基辅酶a (CoA) CoA转移酶基因的新型退化实时PCR方法，可以同时定量不同系统发育的丁酸产生细菌。这一途径存在于人类结肠内重要的丁酸盐产生菌群中，因此该试验估计了微生物群产生丁酸盐的能力。
We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryll-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota.