Transcriptional regulation of c-myc during chemically induced differentiation of HL-60 cultures.
Transcriptional regulation of c-myc during chemically induced differentiation of HL-60 cultures.
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发表时间：
1985-02
期刊：
影响因子：
11.2
通讯作者：
L. Grosso;H. Pitot
中科院分区：
文献类型：
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作者：
L. Grosso;H. Pitot
以前的研究表明，细胞myc同源RNA在HL-60培养物中的水平升高，而在化学诱导HL-60细胞分化后，该信使RNA水平降低。从HL-60细胞中分离的核转录系统被用来调查是否在c-myc基因的转录速率的改变与这种减少myc RNA。在向HL-60培养物中加入180 mM二甲基亚砜或60 nM 12-O-十四烷酰基佛波醇-13-乙酸酯5天后，与对照培养物相比，c-myc基因的转录显著降低。这种特定的减少并不伴随着改变，无论是散装率的转录c-myc拷贝数。这表明细胞myc RNA水平的降低是由于myc原癌基因转录的降低。
Previous studies have demonstrated both an elevated level of cellular myc-homologous RNA in HL-60 cultures and a decrease in this messenger RNA following the chemically induced differentiation of HL-60 cells. A nuclear transcription system isolated from HL-60 cells was used to investigate whether an alteration in the rate of transcription of the c-myc gene was associated with this decrease in myc RNA. Five days after the addition of either 180 mM dimethyl sulfoxide or 60 nM 12-O-tetradecanoylphorbol-13-acetate to HL-60 cultures, transcription of the c-myc gene was markedly reduced when compared with control cultures. This specific decrease was not accompanied by an alteration in either the bulk rate of transcription of the c-myc copy number. This suggests that the decreased cellular myc RNA levels are due to decreased transcription of the myc protooncogene.