Repair of surgical clefts of the hard palate in beagles.
Repair of surgical clefts of the hard palate in beagles.
复制标题
比格犬硬腭裂的手术修复。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
Perry Ht
中科院分区：
文献类型：
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作者：
Forbes Dp;Kaminski Ej;Perry Ht
主要通过监测颅面生长来评估腭裂修复的生物学反应。对于修复后的上颚的再生能力知之甚少。在本研究中，18只比格犬幼崽（51至58天大）被分配到三组之一：（1）对照组，未进行手术； (2) 裂组，8周龄时通过手术形成后硬腭裂（平均骨量：3.1 x 11.7 mm）； (3)修复组，同第2组，12周龄进行软组织闭合。通过头影测量和牙模测量来监测颅面生长。每隔 6 周进行一次记录。产生裂口后16或28周处死动物。进行常规组织学检查和碱性磷酸酶活性的组织化学检测，以检查软组织修复和骨形成的质量和程度。对腭裂组的分析显示，骨裂的尺寸随着时间的推移而增大。组织学检查表明，在 24 周龄（修复后 12 周），骨腭内侧边缘主动减少，破骨细胞活性证明了这一点。 36周龄时，未检测到成骨细胞和破骨细胞活性。 36 周时，骨裂组的骨裂平均尺寸为 7.9 x 18.8 毫米。在修复组中，发生了部分骨修复。然而，在预测修复范围或位置方面没有发现一致性。碱性磷酸酶活性的组织化学检测表明修复组有更多的新骨形成。在一些部位，观察到缝线再生。与骨形成量一样，缝线再生量也是可变的。这项研究表明，裂口的存在会抑制裂口边缘的成骨细胞活性，并且修复后腭的再生潜力有限。
The biological responses to the repair of palatal clefts has been evaluated principally by monitoring craniofacial growth. Little is known about the regenerative ability of the repaired palate. In the present study, 18 Beagle pups (51 to 58 days old) were assigned to one of three groups: (1) control group, having no surgery; (2) cleft group, having a surgically created cleft of the posterior hard palate (mean bony measurement: 3.1 x 11.7 mm) at 8 weeks of age; and (3) repaired group, same as group 2, and followed by soft-tissue closure at 12 weeks of age. Craniofacial growth was monitored by cephalometric and dental cast measurements. Records were taken at 6-week intervals. Animals were sacrificed either 16 or 28 weeks after time of cleft creation. Routine histologic examination and histochemical detection of alkaline phosphatase activity were performed to examine the quality and extent of soft-tissue repair and bone formation. Analysis of the cleft palate group revealed that the size of the bony cleft increased with time. The histologic examination demonstrated at 24 weeks of age (12 weeks after the repair) active reduction of medial margin of the bony palate as evidenced by osteoclastic activity. At 36 weeks of age, neither osteoblastic nor osteoclastic activity was detected. The mean dimensions of the bony cleft, in the cleft group at 36 weeks, were 7.9 x 18.8 mm. In the repaired group, partial bone repair occurred. However, no consistency was seen in predicting extent or location of repair. Histochemical detection of alkaline phosphatase activity indicated that the repaired group had greater amounts of new bone formation. In some sites, suture regeneration was seen. As with the amount of bone formation, the amount of suture regeneration was variable. This study revealed that the presence of a cleft inhibits osteoblastic activity along the margin of the cleft, and there is limited potential for regeneration of the palate subsequent to the repair.