Cloning of a cDNA encoding the thaumatin-like protein of winter rye (Secale cereale L. Musketeer) and its functional characterization
Cloning of a cDNA encoding the thaumatin-like protein of winter rye (Secale cereale L. Musketeer) and its functional characterization
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DOI：
10.1093/jxb/50.339.1627
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发表时间：
1999-10
影响因子：
6.9
通讯作者：
Yee-Wai Chan;W. L. Tung;M. Griffith;K. Chow
中科院分区：
文献类型：
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作者：
Yee-Wai Chan;W. L. Tung;M. Griffith;K. Chow
从冬黑麦（Secale cereale L.）cDNA文库中克隆了一个编码173个氨基酸的开放阅读框架的cDNA克隆WRTLP 2。Musketeer）。从克隆的cDNA推导出的氨基酸序列与其他谷物的胞外和低分子量的thaumatin样蛋白具有非常高的序列相似性（70-95%）。在大肠杆菌中过量表达该cDNA是可能的，并且发现该克隆的编码蛋白对几种真菌菌株表现出抗真菌活性。
A cDNA clone, WRTLP2, encoding an open reading frame of 173 amino acids, was recovered from a cDNA library of winter rye (Secale cereale L. Musketeer). The amino acid sequence deduced from the cloned cDNA exhibits very high sequence similarity (70-95%) with those of extracellular and low molecular weight thaumatin-like proteins of other cereals. It was possible to overexpress this isolated cDNA in Escherichia coli and it was found that the encoded protein of this clone exhibited antifungal activities against several fungal strains.