The contribution of pneumococcal cell wall to the pathogenesis of experimental otitis media.
The contribution of pneumococcal cell wall to the pathogenesis of experimental otitis media.
复制标题
肺炎球菌细胞壁对实验性中耳炎发病机制的贡献。
DOI：
10.1093/infdis/157.2.245
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Tuomanen,E
中科院分区：
文献类型：
--
作者：
Ripley-Petzoldt,ML;Giebink,GS;Juhn,SK;Aeppli,D;Tomasz,A;Tuomanen,E
我们研究了在灰鼠中耳接种灭活的、有包膜的7 F型肺炎链球菌和灭活的、无包膜的R6 S型肺炎链球菌后，肺炎球菌细胞壁对中耳炎发病机制的作用。肺炎球菌;和分离的R6肺炎球菌细胞壁。与盐水接种的耳朵相比，接种有包囊和无包囊肺炎球菌的耳朵在中耳液中具有显著更高浓度的多形核白细胞、单核白细胞和溶菌酶，并产生更多的上皮化生和肉芽组织。接种灭活、未包囊肺炎球菌的中耳液中溶菌酶的平均浓度高于接种包囊肺炎球菌的中耳液中溶菌酶的平均浓度。与对照耳相比，接种肺炎球菌细胞壁的耳的中耳粘骨膜显示出显著更多的多形性白细胞、上皮化生、上皮下充血和肉芽组织。由于在中耳炎的灰鼠模型中，非存活的、未被包囊的肺炎球菌和肺炎球菌细胞壁引起中耳炎症，因此在细菌溶解期间释放的细胞被膜和细胞壁组分可能有助于人类的慢性渗出性中耳炎。
We studied the contribution of pneumococcal cellwall to the pathogenesis of otitis media in chinchillas after middle ear inoculation of killed, encapsulated type 7FStreptococcus pneumoniae; killed, unencapsulated R6S. pneumoniae; and isolated R6 pneumococcal cell wall. Ears inoculated with encapsulated and unencapsulated pneumococci had significantly higher concentrations of polymorphonuclear and mononuclear leukocytes and lysozyme in middle ear fluid and developed more epithelial metaplasia and granulation tissue than did saline-inoculated ears. The mean concentration of lysozyme in middle ear fluid was higher in ears inoculated with killed, unencapsulated than encapsulated pneumococci. The middle ear mucoperiosteum of ears inoculated with pneumococcal cell wall showed significantly more polymorphonuclear leukocytes, epithelial metaplasia, subepithelial congestion, and granulation tissue than did control ears. Because nonviable, unencapsulated pneumococci and pneumococcal cell wall caused middle ear inflammation in the chinchilla model of otitis media, it is possible that cell envelope and cell wall components released during bacterial lysis may contribute to chronic otitis media with effusion in humans.