Neurospora crassa Light Signal Transduction Is Affected by ROS.
Neurospora crassa Light Signal Transduction Is Affected by ROS.
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DOI：
10.1155/2012/791963
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Deryabina YI
中科院分区：
文献类型：
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作者：
Belozerskaya TA;Gessler NN;Isakova EP;Deryabina YI
在子囊菌真菌粗糙脉孢菌蓝紫光控制基因的表达，负责生殖结构的分化，次生代谢产物的合成，和昼夜节律振荡器的活动。脉孢菌细胞中的主要光感受器是WCC，其是由含有PAS结构域的多肽WC-1和WC-2形成的异二聚体复合物，所述多肽是基因白色领-1和白色领-2的产物。光信号转导由LOV结构域（PAS结构域的特化变体）非共价结合的激发的FAD分子的光化学活性启动。WC-1和WC-2蛋白中锌指（GATA识别序列）的存在表明它们可能具有转录因子的功能。然而，光转导机制的关键分析认为，在真菌中缺乏WCC或其同系物时存在残留光响应。所呈现的数据指向由光子刺激产生的内源性ROS，作为将光信号传递到下游靶标的替代输入。
In the ascomycete fungus Neurospora crassa blue-violet light controls the expression of genes responsible for differentiation of reproductive structures, synthesis of secondary metabolites, and the circadian oscillator activity. A major photoreceptor in Neurospora cells is WCC, a heterodimeric complex formed by the PAS-domain-containing polypeptides WC-1 and WC-2, the products of genes white collar-1 and white collar-2. The photosignal transduction is started by photochemical activity of an excited FAD molecule noncovalently bound by the LOV domain (a specialized variant of the PAS domain). The presence of zinc fingers (the GATA-recognizing sequences) in both WC-1 and WC-2 proteins suggests that they might function as transcription factors. However, a critical analysis of the phototransduction mechanism considers the existence of residual light responses upon absence of WCC or its homologs in fungi. The data presented point at endogenous ROS generated by a photon stimulus as an alternative input to pass on light signals to downstream targets.