Detection of Campylobacter rectus in periodontitis sites by monoclonal antibodies
Detection of Campylobacter rectus in periodontitis sites by monoclonal antibodies
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DOI：
10.1034/j.1600-0765.2003.01627.x
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发表时间：
2003-02-01
影响因子：
3.5
通讯作者：
Okuda, K
中科院分区：
文献类型：
--
作者：
Ihara, H;Miura, T;Okuda, K
直弯曲杆菌是一种革兰氏阴性、微需氧、能动的细菌，被认为在人类牙周炎中起致病作用。表面成分，如鞭毛，表面层（S-层）和细胞毒素，已被报道为可能的微生物的毒力因子。在本研究中，制备了针对该细菌表面成分的单克隆抗体，以检测和研究C.直肌在牙周炎中的作用两个单克隆抗体，指定CRT-1和CRT-2，通过免疫印迹分析识别一个特殊的150 kDa的S-层蛋白。CRT-2抗体与所有C.腹直肌菌株，除S层阴性菌株外，其余菌株均为腹直肌[C.腹直肌ATCC 33238 S层（-）菌株]。CRT-3抗体与C.与弯曲杆菌ATCC 51164和CCUG 11641菌株交叉反应。用斑点杂交法，我们能够检测到C。当牙龈下牙菌斑样本中存在少至103种微生物时，使用CRT-2抗体检测直肌。检测C.菌斑标本中直肌与临床发现如探诊深度（P < 0.001）、探诊出血（P < 0.001）和牙龈指数（P < 0.001）显著相关。这些结果表明，C.直肌可能是牙周病状态的重要指标。
Campylobacter rectus, a gram-negative, microaerophilic, and motile bacterium, has been proposed to play a pathogenic role in human periodontitis. Surface components, such as the flagellum, surface layer (S-layer), and cytotoxin, have been reported as possible virulence factors of the microorganism. In the present study, monoclonal antibodies against surface components of this bacterium were produced to detect and investigate the pathogenic potential of C. rectus in periodontitis. Two monoclonal antibodies, designated CRT-1 and CRT-2, recognized a peculiar 150 kDa S-layer protein by immunoblot analysis. The CRT-2 antibody reacted to all C. rectus strains tested, except for the S-layer negative strain of the species [C. rectus ATCC 33238 S-layer (-) strain]. The CRT-3 antibody reacted to a 60-kDa protein in C. rectus and also cross-reacted with Campylobacter showae ATCC 51164 and CCUG 11641 strains. Using the dot-blot method, we were able to detect C. rectus using the CRT-2 antibody when as few as 103 organisms were present in a subgingival dental plaque sample. Detection of C. rectus in plaque samples correlated significantly with clinical findings such as probing depth (P < 0.001), bleeding on probing (P < 0.001), and gingival index (P < 0.001). These findings indicate that infection by C. rectus may be an important indicator of periodontal disease status.