Untargeted metabolomics studies employing NMR and LC-MS reveal metabolic coupling between Nanoarcheum equitans and its archaeal host Ignicoccus hospitalis.
Untargeted metabolomics studies employing NMR and LC-MS reveal metabolic coupling between Nanoarcheum equitans and its archaeal host Ignicoccus hospitalis.
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DOI：
10.1007/s11306-014-0747-6
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发表时间：
2015-08-01
期刊：
影响因子：
--
通讯作者：
Bothner B
中科院分区：
文献类型：
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作者：
Hamerly T;Tripet BP;Tigges M;Giannone RJ;Wurch L;Hettich RL;Podar M;Copié V;Bothner B
种间相互作用是微生物群落形成和传染病的基础。系统生物学能够构建描述这种相互作用的复杂模型，从而更好地了解疾病状态和社区。然而，在理解复杂生物体之间的相互作用之前，必须研究出更简单的现实世界宿主微生物系统的代谢和能量影响。为此，进行了非靶向代谢组学实验，并将其与蛋白质组学数据相结合，以表征两种极端嗜热微生物物种之间的关键分子水平相互作用，这两种微生物物种都具有减少的基因组。利用LC-MS和NMR代谢组学研究了医院古菌和马纳米古菌之间的代谢变化和代谢产物的转移。对这样一个系统的研究是具有挑战性的，因为没有可用的遗传工具，在实验室中的生长是具有挑战性的，并且它们相互作用的机制是未知的。结合鸟枪法蛋白质组学中获得的相对酶水平信息，代谢组学数据为研究I.受N.包括精氨酸、异亮氨酸和CTP生物合成。在生物体水平上，数据表明N.马毒素利用I.以满足其自身的代谢需求。这一发现是基于N.马的消费的一个显着部分的代谢物池在I。hospitalis不能仅仅归因于N.马结合LC-MS和NMR代谢组学数据集提高了代谢组学的覆盖率，并增强了细胞代谢物的鉴定和定量。
Interspecies interactions are the basis of microbial community formation and infectious diseases. Systems biology enables the construction of complex models describing such interactions, leading to a better understanding of disease states and communities. However, before interactions between complex organisms can be understood, metabolic and energetic implications of simpler real-world host-microbe systems must be worked out. To this effect, untargeted metabolomics experiments were conducted and integrated with proteomics data to characterize key molecular-level interactions between two hyperthermophilic microbial species, both of which have reduced genomes. Metabolic changes and transfer of metabolites between the archaea Ignicoccus hospitalis and Nanoarcheum equitans were investigated using integrated LC-MS and NMR metabolomics. The study of such a system is challenging, as no genetic tools are available, growth in the laboratory is challenging, and mechanisms by which they interact are unknown. Together with information about relative enzyme levels obtained from shotgun proteomics, the metabolomics data provided useful insights into metabolic pathways and cellular networks of I. hospitalis that are impacted by the presence of N. equitans, including arginine, isoleucine, and CTP biosynthesis. On the organismal level, the data indicate that N. equitans exploits metabolites generated by I. hospitalis to satisfy its own metabolic needs. This finding is based on N. equitans’s consumption of a significant fraction of the metabolite pool in I. hospitalis that cannot solely be attributed to increased biomass production for N. equitans. Combining LC-MS and NMR metabolomics datasets improved coverage of the metabolome and enhanced the identification and quantitation of cellular metabolites.