Evidence of bluetongue and Epizootic Haemorrhagic disease circulation on the island of Mayotte
Evidence of bluetongue and Epizootic Haemorrhagic disease circulation on the island of Mayotte
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DOI：
10.1016/j.actatropica.2018.12.037
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发表时间：
2019-03-01
期刊：
影响因子：
2.7
通讯作者：
Cetre-Sossah, Catherine
中科院分区：
文献类型：
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作者：
Dommergues, Laure;Viarouge, Cyril;Cetre-Sossah, Catherine
为了解马约特市两种环状病毒：蓝舌病毒(BTV)和流行性出血症病毒(EHDV)的流行情况，进行了横断面研究。在2016年2月至6月期间，总共对385头无症状牛进行了血液采样(每头牛一只EDTA和一只血清试管)。检测抗体(EL ISA)和基因组流行率(PCR)。几乎所有入选牛都有BTV和EHDV抗体，分别为99.5%(CI95%[98.00,100])和96.9%(CI95%[94.5，98.3])。大多数牛在出生后的第一年就获得了抗体。EHDV和BTV基因组分别检出25.2%(CI95%[21.1，29.8])和18.2%(CI95%[14.6，22.4])。BTV和EHDV混合感染占9.4%(CI95%[6.8，12.7])。据报道，三岁以下的牛比年龄较大的牛更容易被报告为基因组检测阳性。从BTV-4、BTV-9、BTV-11、BTV-15、BTV-19和EHDV-6共8份样品中鉴定出5种BTV血清型和1种EHDV血清型，其中一些在邻近地区有报道。BTV和EHDV都在马约特及其周边地区传播。
A cross-sectional study was conducted to explore the epidemiological situation in Mayotte regarding two orbiviruses: Bluetongue virus (BTV) and Epizootic Haemorrhagic Disease virus (EHDV). In all, 385 individual asymptomatic cattle were blood-sampled (one EDTA and one serum tube per animal) between February and June 2016. Antibody (ELISA) and genome prevalence (PCR) was assessed. Almost all the selected cattle showed antibodies against both BTV and EHDV, at 99.5% (CI95% [98.00, 100]) and 96.9% (CI95% [94.5, 98.3]), respectively. Most of the cattle acquired antibodies in their first years of age. EHDV and BTV genomes were detected in 25.2% (CI95% [21.1, 29.8]) and 18.2% (CI95% [14.6, 22.4]) of samples, respectively. Coinfection with BTV and EHDV was observed in 9.4% of samples (CI95% [6.8, 12.7]). Cattle under three years old were more frequently reported as positive for genome detection by PCR than older cattle. Five serotypes of BTV and one serotype of EHDV were identified from eight samples: BTV-4, BTV-9, BTV-11, BTV-15, BTV-19 and EHDV-6, of which some were reported in neighbouring areas. BTV and EHDV both circulate in Mayotte and in its surrounding territories.