TIME COURSE OF DEVELOPMENT OF ENZYMES INVOLVED IN SYNTHESIS OF NOREPINEPHRINE IN SUPERIOR CERVICAL GANGLION OF RAT FROM BIRTH TO ADULT LIFE
TIME COURSE OF DEVELOPMENT OF ENZYMES INVOLVED IN SYNTHESIS OF NOREPINEPHRINE IN SUPERIOR CERVICAL GANGLION OF RAT FROM BIRTH TO ADULT LIFE
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DOI：
10.1016/0006-8993(72)90146-1
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发表时间：
1972-01-01
期刊：
影响因子：
2.9
通讯作者：
KETTLER, R
中科院分区：
文献类型：
--
作者：
THOENEN, H;SANER, A;KETTLER, R
本文研究了大鼠上级颈神经节从出生到75日龄的一般生长及其节前胆碱能和节后肾上腺素能神经元特异性酶的发育。总蛋白质含量，作为一般生长的措施，从出生到成年增加了2.5倍，并在20日龄时达到最大值。胆碱乙酰转移酶的活性增加，这是选择性地位于节前胆碱能神经，这是生物化学相关的突触的形成，达50倍的总和15倍的具体活动。肾上腺素能神经元特有的酶活性在30日龄时达到最大值，但增幅较小。酪氨酸羟化酶的比活性的最大增加仅为40%，并且在出生后10-14天已经达到。多巴胺β-羟化酶比活力增加2.5倍，但在40日龄以前未达到最大值。多巴脱羧酶是参与去甲肾上腺素合成的第三种酶，神经生长因子和节前神经对节后肾上腺素能神经元正常发育的活性的重要性与以前的研究有关，这些研究表明，神经生长因子在新的肾上腺素能神经元中，出生大鼠和成年大鼠增加的节前活动产生肾上腺素能神经元中酪氨酸羟化酶和多巴胺β-羟化酶的选择性诱导。
The general growth of the rat superior cervical ganglion and the development of enzymes specific for its preganglionic cholinergic and postganglionic adrenergic neurons have been studied from birth to 75 days of age. The total protein content, taken as a measure of general growth, increased 2.5-fold from birth to adulthood and approached its maximum at 20 days of age. The increase in the activity of choline acetyltransferase, which is selectively located in preganglionic cholinergic nerves and which is biochemical correlate for the formation of synapses, amounted to 50-fold for total and to 15-fold for specific activity. The maximum was reached at 30 days of age.The increase in the activity of the enzymes characteristics of the adrenergic neurons was much smaller. The maximal increase in the specific activity of tyrosine hydroxylase amounted to only 40% and was already reached 10–14 days after birth. The specific activity of dopamine β-hydroxylase increased 2.5-fold, but the maximum was not reached before 40 days of age. The activity of DOPA decar☐ylase, the third enzymes involved in the synthesis of norepinephrine, increased virtually in parallel with the general growth of the ganglia.The importance of the nerve growth factor and the activity of preganglionic nerves for the normal development of postganglionic adrenergic neurons are discussed in connection with previous studies which showed that both nerve growth factor in new-born rats and increased preganglionic activity in adult rats produce a selective induction of tyrosine hydroxylase and dopamine β-hydroxylase in adrenergic neurons.