Transcriptome and organellar sequencing highlights the complex origin and diversification of allotetraploid Brassica napus
Transcriptome and organellar sequencing highlights the complex origin and diversification of allotetraploid Brassica napus
复制标题
DOI：
10.1038/s41467-019-10757-1
复制
发表时间：
2019-06-28
影响因子：
16.6
通讯作者：
Pires, J. Chris
中科院分区：
文献类型：
--
作者：
An, Hong;Qi, Xinshuai;Pires, J. Chris
甘蓝型油菜是一种异源四倍体作物，被认为是由未知品种的油菜和甘蓝杂交而来的。尽管甘蓝型油菜具有重要的经济价值，但关于它的系统发育关系、遗传结构和多样性仍有许多未解决的问题。在这里，我们对183份甘蓝型油菜(包括油菜籽、甘蓝和西伯利亚甘蓝)、112份甘蓝型油菜和62份甘蓝及其野生近缘的不同种质进行了全面的研究。利用甘蓝型油菜种质资源的RNA-SEQ序列，我们定义了6个遗传类群的遗传多样性和亚基因组变异。甘蓝型油菜及其祖先的核和细胞器系统发育表现出不同的遗传和形成后渐渗模式。我们识别了具有选择性扫描特征的区域，并检测到了8,187个差异表达基因，这意味着甘蓝型油菜的多样化。本研究突出了甘蓝型油菜复杂的起源和进化过程，为甘蓝型油菜育种和种质保存提供了新的思路。
Brassica napus, an allotetraploid crop, is hypothesized to be a hybrid from unknown varieties of Brassica rapa and Brassica oleracea. Despite the economic importance of B. napus, much is unresolved regarding its phylogenomic relationships, genetic structure, and diversification. Here we conduct a comprehensive study among diverse accessions from 183 B. napus (including rapeseed, rutabaga, and Siberian kale), 112 B. rapa, and 62 B. oleracea and its wild relatives. Using RNA-seq of B. napus accessions, we define the genetic diversity and sub-genome variance of six genetic clusters. Nuclear and organellar phylogenies for B. napus and its progenitors reveal varying patterns of inheritance and post-formation introgression. We discern regions with signatures of selective sweeps and detect 8,187 differentially expressed genes with implications for B. napus diversification. This study highlights the complex origin and evolution of B. napus providing insights that can further facilitate B. napus breeding and germplasm preservation.