Distinct replication and gene expression strategies of the Rice Stripe virus in vector insects and host plants
Distinct replication and gene expression strategies of the Rice Stripe virus in vector insects and host plants
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水稻条纹病毒在媒介昆虫和宿主植物中的独特复制和基因表达策略
DOI：
10.1099/jgv.0.001255
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发表时间：
2019
影响因子：
3.8
通讯作者：
Qui Feng
中科院分区：
文献类型：
--
作者：
Zhao Wan;Wang Qianshuo;Xu Zhongtian;Liu Renyi;Qui Feng
持久繁殖植物病毒通常在媒介昆虫和宿主植物之间传播。为了适应两种不同的生物体，病毒可能表现出不同的基因组复制或基因表达模式。为了验证这一假设，我们应用绝对实时定量PCR方法，根据小褐飞虱和水稻植株的感染时间，分别测量和比较了水稻条纹病毒（RSV）4个基因组RNA片段的复制水平和7个基因的表达水平。在媒介昆虫中，RNA3 开始复制的时间晚于其他片段，而 RNA2 在感染过程中几乎保持不变。 RNA1是主要片段，四个片段之间出现了300倍以上的差异。在水稻植株中，四个节段的大小随着感染时间的延长而增大，但在感染后期减小到较低水平。四个部分的比例相差不超过 15 倍。在飞虱中，7种病毒基因在病毒感染过程中观察到了3种表达模式，而在水稻植物中，7种病毒基因的表达模式相似。这些结果反映了持久繁殖植物病毒在适应媒介昆虫和宿主植物时的不同基因组复制和基因表达模式。
Persistent propagative plant viruses are usually transmitted between a vector insect and a host plant. To adapt to the two different organisms, viruses may show distinct genomic replication or gene expression patterns. To verify this hypothesis, we applied an aboslute real-time quantitative PCR method to measure and compare the replication levels of four genomic RNA segments and the expression levels of seven genes of rice stripe virus (RSV) according to the infection time in the small brown planthopper and rice plant, respectively. In the vector insect, RNA3 began replicating later than the other segments, and RNA2 remained nearly constant during the infection process. RNA1 was the dominant segment, and a difference of over 300-fold appeared among the four segments. In rice plants, the size of the four segments increased with infection time, but decreased to a low level in the late infection period. The ratios of the four segments varied by no more than 15-fold. In planthoppers, three expression patterns were observed for the seven viral genes during viral infection, while in rice plants, the expression patterns of the seven viral genes were similar. These results reflect distinct genomic replication and gene expression patterns in a persistent propagative plant virus in adapting to vector insects and host plants.