VvWRKY13 from Vitis vinifera negatively modulates salinity tolerance
VvWRKY13 from Vitis vinifera negatively modulates salinity tolerance
复制标题
来自葡萄的 VvWRKY13 负向调节盐度耐受性
DOI：
10.1007/s11240-019-01620-8
复制
发表时间：
2019-10-15
影响因子：
3
通讯作者：
Liu Xin
中科院分区：
文献类型：
--
作者：
Che Yongmei;Zhang Zhen;Liu Xin
WRKY转录因子参与了多种植物的盐胁迫响应，但其潜在的功能机制还不清楚，特别是在葡萄中。在这项研究中，VvWRKY 13在盐胁迫响应中的作用进行了表征，并探讨了潜在的生理和分子机制。结果表明，盐胁迫诱导了VvWRKY 13基因的表达，且盐胁迫敏感品种的VvWRKY 13转录水平显著高于盐胁迫抗性品种。在拟南芥中过量表达VvWRKY 13显著抑制了盐胁迫下转基因植株的生长，表明VvWRKY 13对盐胁迫反应具有负效应。盐胁迫下，叶片光合速率、脯氨酸和可溶性糖含量、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性、脯氨酸和可溶性糖代谢基因P5 CS 1、SS 1、SS 2和G6 PDH以及SOD和CAT编码基因CAT 1、CAT 2和Cu/Zn-SOD的转录水平均下降，而转基因拟南芥植株中过氧化氢和氧自由基含量比野生型植株增加。VvWRKY 13抑制盐胁迫诱导的SnRK2.3、ABF 1、ABF 2、ABF 3、RD 29 B、RD 29 A和RAB 18的上调，但对SnRK2.2、SnRK2.6或RD 22的转录水平没有显示出显著影响，表明VvWRKY 13通过特异性脱落酸信号传导途径影响盐胁迫耐受性。 VvWRKY 13可能通过特异性阿坝信号传导影响盐胁迫耐受性。
WRKY transcription factors participate in the salt stress responses of various plant species, but the underlying functional mechanisms are not well understood, particularly in grape. In this study, the role of VvWRKY13 in the salt stress response was characterized and the underlying physiological and molecular mechanisms were explored. The results show that VvWRKY13 expression was induced by salt stress, and the VvWRKY13 transcript level in the salt stress-sensitive cultivar was significantly higher than that in the resistant cultivars. Overexpression of VvWRKY13 in Arabidopsis significantly inhibited the growth of transgenic plants under salt stress, indicating that VvWRKY13 has a negative effect on the salt stress response. The photosynthetic rate, contents of proline and soluble sugars, activities of superoxide dismutase (SOD) and catalase (CAT), as well as transcriptional levels of proline and soluble sugar metabolic genes, such as P5CS1, SS1, SS2 and G6PDH, as well as SOD and CAT encoding genes CAT1, CAT2, and Cu/Zn-SOD, all decreased under salt stress, whereas hydrogen peroxide and oxygen-free radical contents increased in transgenic Arabidopsis plants compared with those of wild-type plants. VvWRKY13 suppressed upregulation of SnRK2.3, ABF1, ABF2, ABF3, RD29B, RD29A, and RAB18 induced by salt stress, but showed no significant effect on the transcript levels of SnRK2.2, SnRK2.6, or RD22, indicating that VvWRKY13 affects salt stress tolerance via a specific abscisic acid signaling pathway. VvWRKY13 may affects salt stress tolerance by specific ABA signalling.