Parylene-C Coating Protects Resin-3D-Printed Devices from Material Erosion and Prevents Cytotoxicity toward Primary Cells.
Parylene-C Coating Protects Resin-3D-Printed Devices from Material Erosion and Prevents Cytotoxicity toward Primary Cells.
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Parylene-C 涂层可保护树脂 3D 打印设备免受材料侵蚀，并防止对原代细胞产生细胞毒性。
DOI：
10.1021/acsabm.3c00444
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发表时间：
2023
影响因子：
4.7
通讯作者：
Pompano,RebeccaR
中科院分区：
文献类型：
--
作者：
Musgrove,HannahB;Cook,SophieR;Pompano,RebeccaR
树脂3D打印对于快速制造微型细胞培养装置具有吸引力，但常见的树脂材料在培养条件下不稳定且具有细胞毒性。诸如浸出或过度固化的策略不足以保护敏感的原代细胞，如白色血细胞。在这里，我们评估了使用市售透明树脂的聚对二甲苯C涂层防止细胞毒性浸出、微流体装置降解和小分子吸收的有效性。我们发现，聚对二甲苯C显著改善了与原代小鼠白色血细胞的细胞相容性和印刷品的材料完整性，同时保持了透明树脂所具有的良好光学品质。
Resin 3D printing is attractive for the rapid fabrication of microscale cell culture devices, but common resin materials are unstable and cytotoxic under culture conditions. Strategies such as leaching or overcuring are insufficient to protect sensitive primary cells such as white blood cells. Here, we evaluated the effectiveness of using a parylene C coating of commercially available clear resins to prevent cytotoxic leaching, degradation of microfluidic devices, and absorption of small molecules. We found that parylene C significantly improved both the cytocompatibility with primary murine white blood cells and the material integrity of prints while maintaining the favorable optical qualities held by clear resins.