Chromosome complement of the fungal plant pathogen Fusarium graminearum based on genetic and physical mapping and cytological observations
Chromosome complement of the fungal plant pathogen Fusarium graminearum based on genetic and physical mapping and cytological observations
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DOI：
10.1534/genetics.105.044842
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发表时间：
2005-11-01
期刊：
影响因子：
3.3
通讯作者：
Kistler, HC
中科院分区：
文献类型：
--
作者：
Gale, LR;Bryant, JD;Kistler, HC
构建了丝状真菌禾谷镰刀菌(核形态：赤霉菌)的遗传图谱，以验证和扩大菌株PH-1的全基因组序列组装草案。从测序菌株(PH-1NRRL 31084，最初从密歇根州分离)和来自明尼苏达州的野生菌(00-676，NRRL 34097)的突变体之间建立作图群体。对子囊孢子后代进行了235个座位的分离分析。遗传标记包括序列标记的位点，主要检测为dCAP或CAPS(n=131)和VNTRs(n=31)，此外还有AFLP(n=66)和7个其他标记。虽然大多数标记表现为孟德尔遗传，但观察到25个以聚类为主的标记存在分离扭曲。使用Kosambi映射函数，使用LOD阈值1.5来生成连锁图。检测到9个连锁群，覆盖1234 cM，锚定了草稿序列组装的99.83%。序列组装中的9个连锁群和22个锚定支架可以组装成4条染色体，只剩下5个较小的支架(总共59,630bp)没有锚定核DNA。经细胞学核型分析证实染色体数目为4。对遗传图谱数据的进一步分析发现，不同基因组区域的重组率存在差异，这些差异往往跨越数百个千碱基。
A genetic map of the filamentous fungus Fusarium graminearum (teleomorph: Gibberella zeae) was constructed to both validate and augment the draft whole-genome sequence assembly of strain PH-1. A mapping population was created from a cross between Mutants of the sequenced strain (PH-1 NRRL 31084, originally isolated from Michigan) and a field strain from Minnesota (00-676, NRRL 34097). A total of I I I ascospore progeny were analyzed for segregation at 235 loci. Genetic markers consisted of sequence-tagged sites, primarily detected as dCAPS or CAPS (n = 131) and VNTRs (n = 31), in addition to AFLPs (n = 66) and 7 other markers. While most markers exhibited Mendelian inheritance, segregation distortion was observed for 25 predominantly clustered markers. A linkage map was generated using the Kosambi mapping function, using a LOD threshold value of 1.5. Nine linkage groups were detected, covering 1234 cM and anchoring 99.83% of the draft sequence assembly. The nine linkage groups and the 22 anchored scaffolds from the sequence assembly could be assembled into four chromosomes, leaving only five smaller scaffolds (59,630bp total) of the nuclear DNA unanchored. A chromosome number of four was confirmed by cytological karyotyping. Further analysis of the genetic map data identified variation in recombination rate in different genomic regions that often spanned several hundred kilobases.