PNA interference mapping demonstrates functional domains in the noncoding RNA Xist
PNA interference mapping demonstrates functional domains in the noncoding RNA Xist
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DOI：
10.1073/pnas.161173098
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发表时间：
2001-07-31
影响因子：
11.1
通讯作者：
Strauss, WM
中科院分区：
文献类型：
--
作者：
Beletskii, A;Hong, YK;Strauss, WM
非编码RNA Xist已被证明是X染色体失活所必需的，并覆盖失活的X染色体（Xi）。因此，理解Xi形成的一个重要问题是Xist的结合反应是否是X染色体失活所必需的。在这篇文章中，我们证明了失败的X染色体沉默，如果协会的Xist与X染色体被抑制。染色质结合区的功能映射和评估，通过使用的方法，我们称之为肽核酸（PNA）干扰映射研究非编码RNA在活细胞中的功能。在所报道的实验中，针对Xist RNA的特定区域的单个19-bp反义细胞渗透PNA引起Xi的破坏。Xi与宏组蛋白H2 A的缔合也受到PNA干扰作图的干扰。
The noncoding RNA Xist has been shown to be essential for X-chromosome inactivation and to coat the inactive X-chromosome (Xi). Thus, an important question in understanding the formation of Xi is whether the binding reaction of Xist is necessary for X-chromosome inactivation. In this article, we demonstrate the failure of X-chromosome silencing if the association of Xist with the X-chromosome is inhibited. The chromatin-binding region was functionally mapped and evaluated by using an approach for studying noncoding RNA function in living cells that we call peptide nucleic acid (PNA) interference mapping. In the reported experiments, a single 19-bp antisense cell-permeating PNA targeted against a particular region of Xist RNA caused the disruption of the Xi. The association of the Xi with macro-histone H2A is also disturbed by PNA interference mapping.