Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line
Inhibition of Siniperca chuatsi Rhabdovirus by RNA Interference in a Fish Cell Line
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RNA干扰对鱼细胞系中鳜鱼弹状病毒的抑制作用
DOI：
10.3147/jsfp.47.30
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发表时间：
2012-03-01
期刊：
影响因子：
0.6
通讯作者：
Zhang, Qi-Ya
中科院分区：
文献类型：
--
作者：
Zhou, Guang-Zhou;Zhu, Rong;Zhang, Qi-Ya
我们在鱼类细胞系EPC中评价了几种对应于中华鲟弹状病毒（Siniathuatsi rhabdovirus，SCRV）核蛋白（Nucleoprotein，N）基因的小干扰RNA（small interfering RNA，siRNAs）对SCRV的抗病毒活性。在我们检查的siRNA中，与模拟组相比，si983在降低病毒滴度方面最有效。Western blot分析表明，si983以剂量依赖方式抑制N基因表达和病毒复制。这些结果表明，N基因983位点是抗病毒治疗的潜在靶点。此外，靶向N基因的RNAi可能代表一种可行的治疗SCRV感染的方法。
We evaluated the antiviral activity of several small interfering RNAs (siRNAs) corresponding to the Siniperca chuatsi rhabdovirus (SCRV) nucleoprotein (N) gene against the SCRV in a fish cell line, EPC. Among the siRNAs we examined, si983 was most effective at reducing virus titer compared to the mock group. Western blot analysis suggested that si983 inhibited N gene expression and virus replication in a dose-dependent manner. These results indicate that N gene 983 sites are potential targets for antiviral therapy. Furthermore, RNAi targeting of the N gene may represent a viable method of treating against SCRV infection.