Western blot analysis of Amblyomma americanum-derived stage-specific and shared antigens using serum from guinea pigs expressing resistance.
Western blot analysis of Amblyomma americanum-derived stage-specific and shared antigens using serum from guinea pigs expressing resistance.
复制标题
使用表达抗性的豚鼠血清对美洲钝眼蝽衍生的阶段特异性和共享抗原进行蛋白质印迹分析。
DOI：
10.1016/0304-4017(88)90028-3
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发表时间：
1988
影响因子：
2.6
通讯作者：
Brown,SJ
中科院分区：
文献类型：
--
作者：
Brown,SJ
在Western印迹分析中使用了对幼虫、若虫和成虫Amblyomma americpenditicks具有抗性的豚鼠血清，以鉴定来自卵、幼虫和若虫以及雌性唾液腺提取物制剂的潜在抗原。结果表明，每个生命阶段都有多种独特的抗原，以及三个生命阶段之间的几种共享蛋白质。然而，似乎25和38 kDa的两种特定蛋白质可能比其他蛋白质更重要，这是基于它们在该测定中相对于其他多肽的识别率和强度。
Serum from guinea pigs expressing resistance to larval, nymphal and adultAmblyomma americanumticks was used in Western blot analyses to identify potential antigens from egg, larval and nymphal, and female salivary gland extract preparations. The results demonstrate multiple antigens unique to each life stage, as well as several shared proteins between the three life stages. However, it appears as if two particular proteins of 25 and 38 kDa may be more important than others, based upon their prevalence and intensity of recognition in this assay relative to other polypeptides.