Continued expression of the chicken caudal homologue in endodermally derived organs.
Continued expression of the chicken caudal homologue in endodermally derived organs.
复制标题
鸡尾同源物在内胚层衍生的器官中持续表达。
作者：
U. Doll;J. Niessing
我们发现 CHox-cad 基因在从发育第 1 天到第 14 天的整个胚胎发生过程中持续表达。在成年鸡中，CHox-cad 似乎仅在肠道中表达。基因组 Southern 印迹杂交实验表明，每个单倍体基因组可能只有一个 CHox-cad 基因，由该基因转录 2.9 和 1.6 kb 的 RNA。使用 CHox-cad 探针对 80 小时至 12 天的鸡胚切片进行原位杂交表明，该基因在内胚层来源的器官中高水平表达：发育中的肝脏、胰腺、肺和整个肠道的上皮衬里。到发育第 16 天，在肠道上皮层中观察到 CHox-cad 信号，但在其他内胚层衍生器官中不再观察到。 CHox-cad 表达与这些内胚层衍生器官的发育开始同时发生，可能表明该基因在器官发生的早期步骤中发挥着重要作用。
We show that the CHox-cad gene is continuously expressed throughout embryogenesis from Days 1 to 14 of development. In the adult chicken, CHox-cad appears to be expressed exclusively in the gut. Genomic Southern blot hybridization experiments suggest that there may be only a single CHox-cad gene per haploid genome from which RNAs of 2.9 and 1.6 kb are transcribed. In situ hybridization to sections of 80-hr to 12-day chick embryos with a CHox-cad probe reveals that this gene is expressed at high levels in organs of endodermal origin: the developing liver, pancreas, lung, and in the epithelial lining of the entire intestine. By Day 16 of development, CHox-cad signals are observed in the epithelial lining of the gut but no longer in the other endodermally derived organs. The coincidence of CHox-cad expression with the onset of development of these endoderm-derived organs may indicate an important role for that gene during the early steps of organogenesis.