Cell migration and activated PI3K/AKT-directed elongation in the developing rat Mullerian duct
Cell migration and activated PI3K/AKT-directed elongation in the developing rat Mullerian duct
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DOI：
10.1016/j.ydbio.2008.10.027
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发表时间：
2009-01-15
影响因子：
2.7
通讯作者：
Donahoe, Patricia K.
中科院分区：
文献类型：
--
作者：
Fujino, Akihiro;Arango, Nelson A.;Donahoe, Patricia K.
在脊椎动物中，苗勒管沿着沃尔夫管伸长，沃尔夫管是苗勒管形成所需的中肾结构。最近，已经确定了初始苗勒管形成所需的几个基因。然而，穆勒管伸长的精确机制仍有待阐明。在这项研究中，我们探讨了动态形态学变化的拉长苗勒管在大鼠泌尿生殖嵴器官培养微切口和/或化学抑制剂操作。发育中的苗勒管的机械分裂表明，苗勒管上皮细胞积极迁移沿着前后轴独立的导管的前部的增殖扩张。我们发现PI 3 K/AKT信号通路在苗勒管上皮中被激活，并且是导管尖端伸长所需的;然而，当PI 3 K/AKT失活时，苗勒管上皮细胞在尖端附近的迁移保持完整。虽然我们对导致苗勒管退化的分子和细胞机制了解很多，但本研究结果提供了对苗勒管堡垒形成机制和早期小管形成一般过程的更全面理解。(C)2008年由Elsevier Inc.出版
In vertebrates, the Mullerian duct elongates along the Wolffian duct, a mesonephric structure that is required for Mullerian duct formation. Recently, several genes required for initial Mullerian duct formation have been identified. However, the precise mechanism of Mullerian duct elongation remains to be elucidated. In this Study, we investigated dynamic morphological changes in the elongating Mullerian duct in rat urogenital ridges in organ culture manipulated by microincision and/or chemical inhibitors. Mechanical division of the developing Mullerian duct showed that epithelial cells of the Mullerian duct actively migrate along the anterior-posterior axis independent of the proliferative expansion of the anterior portion of the duct. We found that the PI3K/AKT signaling pathway is activated in the Mullerian duct epithelium and is required for elongation of the tip of the duct; however, migration of Mullerian duct epithelial cells proximal to the tip remains intact when PI3K/AKT is inactivated. Although much is known about the molecular and cellular mechanisms leading to Mullerian duct regression, the present findings provide a fuller understanding of the mechanisms contributing to Mullerian duct fort-nation and to the general process of early tubulogenesis. (C) 2008 Published by Elsevier Inc.