Formation of a mandibular condyle in vitro by tissue engineering
Formation of a mandibular condyle in vitro by tissue engineering
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DOI：
10.1053/joms.2003.50015
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发表时间：
2003-01-01
影响因子：
1.9
通讯作者：
Troulis, MJ
中科院分区：
文献类型：
--
作者：
Abukawa, H;Terai, H;Troulis, MJ
目的：下颌骨重建过程中经常会产生严重的供区并发症。最近，使用微创技术进行下颌骨重建的报告，降低发病率在主要手术部位。迄今为止，这些技术还没有解决移植物供体部位。我们假设，组织工程技术可用于制造骨，从而消除供体部位morbidity.Methods：猪间充质干细胞（pMSCs）从3尤卡坦小型猪的骨髓中分离出来，并在标准培养瓶中生长。当它们接近融合时，将细胞分离并加入成骨补充剂重新铺板。制备猪下颌骨髁状突模型，并用于制备由可生物降解的聚（DL）-乳酸-羟基乙酸共聚物（PLGA）制成的多孔聚合物支架。将分化的成骨细胞转移到PLGA支架上，并在旋转透氧生物反应器系统中培养6周。通过大体、放射学和组织学检查对由支架和细胞组成的培养结构进行评价。结果：工程化结构呈白色，坚硬，形状与模型髁状突非常相似。X线平片显示，该结构的放射密度介于正常髁突和对照支架之间。组织学上，骨被观察到的PLGA支架的平均厚度为0.03毫米的整个表面上。骨没有观察到在控制scaffolds.Conclusion：在这个初步研究中，自体组织工程骨构建成功地结合生物可降解聚合物和pMSCs。(C)2003年美国口腔颌面外科医师协会。
Purpose: Mandibular reconstructive procedures often produce significant donor site morbidity. Recently, the use of minimally invasive techniques has been reported for mandibular reconstruction with decreased morbidity at the primary operative site. To date, these techniques have not addressed the graft donor site. We hypothesize that tissue-engineering techniques may be used to fabricate bone and thereby eliminate donor site morbidity.Methods: Porcine mesenchymal stem cells (pMSCs) were isolated from the bone marrow of 3 Yucatan minipigs and grown in standard culture flasks. When they became near-confluent, cells were detached and replated with the addition of osteogenic supplements. A model of a porcine mandibular condyle was made and used to fabricate porous polymer scaffolds from biodegradable Poly (DL)-lactic-co-glycolic acid (PLGA). Differentiated osteoblasts were transferred to the PLGA scaffold and cultured for 6 weeks in a rotational oxygen-permeable bioreactor system. The cultured constructs, consisting of scaffold and cells, were evaluated by gross, radiologic, and histologic examinations.Results: The engineered constructs were white and hard and had a shape that closely resembled that of the model condyle. Plain radiographs demonstrated that the radiodensity of the construct was between that of the normal condyle and that of control scaffolds. Histologically, bone was observed on the entire surface of the PLGA scaffolds with an average thickness of 0.03 mm. Bone was not observed in the control scaffolds.Conclusion: in this pilot study, autologous tissue-engineered bone constructs were successfully made by combining biodegradable polymers and pMSCs. (C) 2003 American Association of Oral and Maxillofacial Surgeons.