Relative overexpression of collagen type I and collagen type III messenger ribonucleic acids by uterine leiomyomas during the proliferative phase of the menstrual cycle.
Relative overexpression of collagen type I and collagen type III messenger ribonucleic acids by uterine leiomyomas during the proliferative phase of the menstrual cycle.
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DOI：
10.1210/jcem.79.3.8077380
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发表时间：
1994-09
期刊：
影响因子：
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通讯作者：
Elizabeth A. Stewart;Andrew J. Friedman;Kimberly Peck;Romana A. Nowak
中科院分区：
文献类型：
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作者：
Elizabeth A. Stewart;Andrew J. Friedman;Kimberly Peck;Romana A. Nowak
子宫平滑肌瘤含有丰富的细胞外基质（ECM）。我们的特点定位的三个ECM蛋白，胶原蛋白I型，胶原蛋白III型，和纤连蛋白，在平滑肌瘤和相邻的正常子宫肌层。我们进一步检测了这些蛋白质的信使核糖核酸（mRNA）水平的表达，这些蛋白质来自1）处于月经周期不同阶段的妇女和2）同一患者的多发性平滑肌瘤。免疫组化染色显示纤维连接蛋白主要分布在平滑肌细胞周围。I型胶原分布在ECM和SMC的细胞质中。III型胶原主要存在于ECM中。与相应的正常子宫肌层相比，平滑肌瘤对I型和III型胶原蛋白的染色更强烈。北方印迹和光密度分析显示，在月经周期的增殖期患者中，相对于邻近的子宫肌层，I型和III型胶原mRNA在平滑肌瘤中持续升高（两者均P < 0.02）。这些差异在分泌期不明显。纤连蛋白mRNA水平变化很大，在月经周期的任何阶段，两种组织之间没有显着差异。我们的结论是，平滑肌瘤显示相对于正常子宫肌层的I型和III型胶原mRNA水平增加。然而，这种差异仅在月经周期的增殖期表现出来。这些发现表明，平滑肌瘤可能是更敏感的激素变化发生在月经周期比正常的子宫肌层。
Uterine leiomyomas contain abundant quantities of extracellular matrix (ECM). We characterized the localization of three ECM proteins, collagen type I, collagen type III, and fibronectin, in leiomyomas and adjacent normal myometrium. We further examined the expression of messenger ribonucleic acid (mRNA) levels for these proteins from 1) women who were in various stages of the menstrual cycle and 2) multiple leiomyomas from the same patient. Immunohistochemical staining showed that fibronectin was localized primarily around individual smooth muscle cells (SMC). Collagen type I was distributed across the ECM and also in the cytoplasm of SMC. Collagen type III was found primarily in the ECM. Leiomyomas showed more intense staining for collagen types I and III than corresponding normal myometrium. Northern blot and densitometric analysis showed that both collagen type I and III mRNAs were consistently elevated in leiomyomas relative to the adjacent myometrium in patients who were in the proliferative phase of the menstrual cycle (P < 0.02 for both). These differences were not evident in the secretory phase. Fibronectin mRNA levels were quite variable, and there were no significant differences between the two tissues at any stage of the menstrual cycle. We conclude that leiomyomas show increased levels of mRNA for collagen types I and III relative to the normal myometrium. However, this difference is only manifested during the proliferative phase of the menstrual cycle. These findings suggest that leiomyomas may be more sensitive to the hormonal changes that occur during the menstrual cycle than is normal myometrium.