Gene expression in the embryonic Xenopus liver
Gene expression in the embryonic Xenopus liver
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DOI：
10.1016/s0925-4773(01)00341-0
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发表时间：
2001-05-01
影响因子：
2.6
通讯作者：
Mason, J
中科院分区：
文献类型：
--
作者：
Zorn, AM;Mason, J
近年来，在揭示非洲爪蟾内胚层特化的分子基础方面取得了重大进展。然而，人们对内胚层的模式化以及内胚层衍生的器官（如肝脏）是如何形成的知之甚少。由于缺乏良好的推定肝组织的分子标记物，进展受到阻碍。在这里，我们检测了肝脏器官形成过程中一些标记基因的胚胎表达，包括转录因子hex、sox 17 alpha和hnf 3 beta，以及一些成人肝脏特异性蛋白质。有趣的是，sox 17 α似乎特异性地标记胆囊前体。在发育7天时，肝分化标志物白蛋白、α 1-微球蛋白/bikunin前体、纤维蛋白原、转铁蛋白和甲状腺素运载蛋白的表达限于分化中的肝芽。然而，令人惊讶的是，在发育3天时，这些基因中的大多数具有更广泛的内胚层表达模式。除了在未分化的肝芽中表达外，它们在整个假定的肠组织中广泛表达，这可能反映了肝脏基因程序如何发育调节的一些一般特征。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
In recent years, significant progress has been made in uncovering the molecular basis of endoderm specification in Xenopus. Much less is understood, however, about endodermal patterning and how endoderm-derived organs such as the liver are formed. Progress has been hampered by the lack of good molecular markers of presumptive liver tissue. Here, we have examined the embryonic expression of a number of marker genes during liver organogenesis, including the transcription factors hex, sox17 alpha, and hnf3 beta, as well as a number of proteins specific to the adult liver. Interestingly, sox17 alpha appears to specifically mark the gall bladder precursors. At 7 days of development expression of the liver differentiation markers albumin, alpha 1-microglobulin/bikunin precursor, fibrinogen, transferrin and transthyretin is restricted to the differentiating liver bud. Surprisingly, however, at 3 days of development most of these genes have a more widespread endodermal expression pattern. In addition to expression in the undifferentiated liver bud they were expressed extensively throughout the presumptive intestinal tissue, which may reflect some general feature of how the hepatic gene program is developmentally regulated. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.