Two subunits specific to the PBAP chromatin remodeling complex have distinct and redundant functions during Drosophila development
Two subunits specific to the PBAP chromatin remodeling complex have distinct and redundant functions during Drosophila development
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DOI：
10.1128/mcb.00747-08
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发表时间：
2008-09-01
影响因子：
5.3
通讯作者：
Treisman, Jessica E.
中科院分区：
文献类型：
--
作者：
Carrera, Ines;Zavadil, Jiri;Treisman, Jessica E.
染色质重塑复合物控制DNA结合位点对转录调节因子的可用性。SWI/SNF类复合物的两种不同的保守形式的特征在于存在特定的辅助亚基。在果蝇中，核心的梵天复合体与Osa结合形成BAP复合体，或与Bap 170和Bap 180结合形成PBAP复合体。OSA突变仅复制由核心复合物亚基突变引起的发育表型的子集。为了测试PBAP复合物是否执行剩余的功能，我们在bap 170和bap 180中产生了突变。令人惊讶的是，我们发现，Bap 180是不是必需的生存能力，虽然它是所需的卵泡细胞正常蛋壳发育。Bap 170是稳定Bap 180蛋白所必需的，但保留这种功能的突变形式足以维持生存和生育能力。这两个亚基的行为冗余，允许变态;使用基因表达谱的bap 170 bap 180双突变体，我们发现，PBAP复合物调节基因参与组织重塑和免疫系统功能。最后，我们产生的突变体缺乏Bap 170，Bap 180，和Osa的种系证明，核心梵天复杂的卵子发生功能，没有任何这些辅助亚基。
Chromatin remodeling complexes control the availability of DNA binding sites to transcriptional regulators. Two distinct conserved forms of the SWI/SNF class of complexes are characterized by the presence of specific accessory subunits. In Drosophila, the core Brahma complex associates either with Osa to form the BAP complex or with Bap170 and Bap180 to form the PBAP complex. osa mutations reproduce only a subset of the developmental phenotypes caused by mutations in subunits of the core complex. To test whether the PBAP complex performs the remaining functions, we generated mutations in bap170 and bap180. Surprisingly, we found that Bap180 is not essential for viability, although it is required in ovarian follicle cells for normal eggshell development. Bap170 is necessary to stabilize the Bap180 protein, but a mutant form that retains this function is sufficient for both survival and fertility. The two subunits act redundantly to allow metamorphosis; using gene expression profiling of bap170 bap180 double mutants, we found that the PBAP complex regulates genes involved in tissue remodeling and immune system function. Finally, we generated mutants lacking Bap170, Bap180, and Osa in the germ line to demonstrate that the core Brahma complex can function in oogenesis without any of these accessory subunits.