Microcantilever-based biosensors
Microcantilever-based biosensors
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DOI：
10.1016/s0304-3991(99)00145-x
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发表时间：
2000-02-01
期刊：
影响因子：
2.2
通讯作者：
Welland, ME
中科院分区：
文献类型：
--
作者：
Moulin, AM;O'Shea, SJ;Welland, ME
研究了基于表面应力的传感器作为生化传感器的应用。原则上，生化物质吸附在微细加工悬臂梁的功能化表面上会引起表面应力，从而导致悬臂梁弯曲。介绍了两个应用：首先，通过测量脂蛋白在糖(肝素)上的吸附所涉及的表面应力，区分了导致胆固醇在动脉中积累的脂蛋白及其氧化形式；其次，监测了由表面诱导的蛋白质构象变化引起的表面应力。这为长时间尺度的地表过程提供了实验证据。(C)2000 Elsevier Science B.V.保留所有权利。
The use of surface stress-based sensors as bio-chemical sensors was investigated. In principle, adsorption of biochemical species on a functionalised surface of a microfabricated cantilever will cause surface stress and consequently the cantilever bends. Two applications are presented: first lipoproteins and their oxidised form which are responsible for cholesterol accumulation in arteries were differentiated by measuring the surface stress involved in their adsorption on a sugar (heparin); secondly, the surface stress resulting from surface induced conformational changes in protein was monitored. That provided experimental evidence of long time-scale surface processes. (C) 2000 Elsevier Science B.V. All rights reserved.