β-Criptoxanthin stimulates bone formation and inhibits bone resorption in tissue culture in vitro
β-Criptoxanthin stimulates bone formation and inhibits bone resorption in tissue culture in vitro
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β-Criptoxanthin 在体外组织培养中刺激骨形成并抑制骨吸收
DOI：
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发表时间：
2004
影响因子：
4.3
通讯作者：
S. Uchiyama
中科院分区：
文献类型：
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作者：
M. Yamaguchi;S. Uchiyama
大量存在于水果中的β-隐黄质对骨代谢的影响至今尚未阐明。采用组织培养法研究了β-隐黄素对骨形成和骨吸收的影响。大鼠股骨骨干（皮质骨）和干骺端（骨小梁）组织培养48小时的Dulbecco的改良Eagle培养基（高葡萄糖，4.5%）补充抗生素和牛血清白蛋白。实验培养物含10 ~（-8）~ 10 ~（-5）M β-隐黄素。β-隐黄素（10-6或10-5 M）的存在导致骨干和干骺端组织中钙含量、碱性磷酸酶活性和脱氧核糖核酸（DNA）含量显著增加。这些增加被完全阻止在放线菌酮（10-6 M），蛋白质合成的抑制剂的存在下。β-胡萝卜素（10-6或10-5 M）或xantine（10-6或10-5 M）对骨干和干骺端钙含量没有影响。骨吸收因子甲状旁腺激素（1-34）（PTH; 10-7 M）或前列腺素E2（PGE 2; 10-5 M）导致骨干和干骺端组织中钙含量显著降低。β-隐黄素（10-8−10-6 M）可完全抑制PTH或PGE 2引起的骨钙含量降低。此外，β-隐黄素（10-8−10-6 M）完全抑制PTH（10-7 M）或PGE 2（10-5 M）诱导的骨干和干骺端组织中葡萄糖消耗和乳酸产生的增加。β-隐黄素（10-7 M）对PTH（10 -7 M）或PGE 2（10-5 M）刺激的骨干钙含量降低的抑制作用在10-3 M钒酸盐（一种蛋白酪氨酸磷酸酶抑制剂）存在下被显著阻止。钒酸盐（10-3 M）对对照骨组织中的钙含量和乳酸产生没有显著影响。本研究表明，β-隐黄素在体外组织培养中对骨形成有直接的促进作用，对骨吸收有抑制作用。
The effect of β-cryptoxanthin, which is greatly present in fruits, has not been clarified so far on bone metabolism. The effect of β-cryptoxanthin on bone formation and bone resorption was investigated in tissue culture in vitro. Rat femoral-diaphyseal (cortical bone) and -metaphyseal (trabecular bone) tissues were cultured for 48 h in Dulbecco's modified Eagle's medium (high glucose, 4.5%) supplemented with antibiotics and bovine serum albumin. The experimental cultures contained 10–8–10–5 M β-cryptoxanthin. The presence of β-cryptoxanthin (10–6 or 10–5 M) caused a significant increase in calcium content, alkaline phosphatase activity and deoxyribonucleic acid (DNA) content in the diaphyseal and metaphyseal tissues. These increases were completely prevented in the presence of cycloheximide (10–6 M), an inhibitor of protein synthesis. β-Carotene (10–6 or 10–5 M) or xantine (10–6 or 10–5 M) had no effect on the diaphyseal and metaphyseal calcium contents. The bone-resorbing factors parathyroid hormone (1–34) (PTH; 10–7 M) or prostaglandin E2 (PGE2; 10–5 M) caused a significant decrease in calcium content in the diaphyseal and metaphyseal tissues. The decrease in bone calcium content induced by PTH or PGE2 was completely inhibited by β-cryptoxanthin (10–8−10–6 M). In addition, β-cryptoxanthin (10–8−10–6 M) completely inhibited the PTH (10–7 M)- or PGE2 (10–5 M)-induced increase in medium glucose consumption and lactic acid production by diaphyseal and metaphyseal tissues. The inhibitory effect of β-cryptoxanthin (10–7 M) on PTH (10–7 M)- or PGE2 (10–5 M)-stimulated decrease in the diaphyseal calcium content was significantly prevented in the presence of 10–3 M vanadate, an inhibitor of protein tyrosine phosphatase. Vanadate (10–3 M) did not have a significant effect on calcium content and lactic acid production in control bone tissues. The present study demonstrates that β-cryptoxanthin has a direct stimulatory effect on bone formation and an inhibitory effect on bone resorption in tissue culture in vitro.
影响因子：
4.1
作者：
Aoki, K;Didomenico, E;Baron, R
通讯作者：
Baron, R
比较合成人甲状旁腺激素（PTH）-（1-34）恶性肿瘤相关肽和牛PTH-（1-34）对器官培养中骨形成和吸收的影响。
影响因子：
4.8
作者：
Klein-Nulend,J;Fall,PM;Raisz,LG
通讯作者：
Raisz,LG