Emissive and Cell-Permeable 3-Pyridyl- and 3-Pyrazolyl-4-azaxanthone Lanthanide Complexes and Their Behaviour in cellulo
Emissive and Cell-Permeable 3-Pyridyl- and 3-Pyrazolyl-4-azaxanthone Lanthanide Complexes and Their Behaviour in cellulo
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DOI：
10.1002/hlca.200900122
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发表时间：
2009-01-01
影响因子：
1.8
通讯作者：
Lamarque, Laurent
中科院分区：
文献类型：
--
作者：
Montgomery, Craig P.;New, Elizabeth J.;Lamarque, Laurent
合成了一系列7种发光Eu(III)和Tb(III)配合物，在多齿大环配体中加入了3-吡啶-4-氮杂黄酮或3-吡唑基-4-氮杂黄酮敏化部分。由于N，N‘-螯合增敏剂的置换，在蛋白质或某些含氧阴离子的存在下，水介质中的高总发射量子产率被衰减。然而，这些复合体被带入细胞，并倾向于在最初的几个小时内定位在线粒体中，然后被运输到内体隔间。细胞摄取研究，在竞争性抑制物或明确定义的摄取途径的促进剂存在的情况下，揭示了一个共同的摄取机制，涉及大吞噬作用。
A series of seven emissive europium(III) and terbium(III) complexes was prepared, incorporating a 3-pyridyl-4-azaxanthone or 3-pyrazolyl-4-azaxanthone sensitising moiety within a polydentate macrocyclic ligand. High overall emission quantum yields in aqueous media are attenuated in the presence of protein or certain oxy anions due to displacement of the N,N'-chelated sensitiser. Nevertheless, these complexes are taken into cells and tend to localise over the first few hours in mitochondria before being trafficked to endosomal compartments. Cell uptake studies, in the presence of competitive inhibitors or promoters of well-defined uptake pathways, reveal a common uptake mechanism involving macro-pinocytosis.