p53-independent transient p21WAF1/CIP1 mRNA induction in the rat brain following experimental traumatic injury
p53-independent transient p21WAF1/CIP1 mRNA induction in the rat brain following experimental traumatic injury
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DOI：
10.1097/00001756-200007140-00003
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发表时间：
2000-07-14
期刊：
影响因子：
1.7
通讯作者：
Yamada, K
中科院分区：
文献类型：
--
作者：
Katano, H;Masago, A;Yamada, K
应用原位杂交技术研究了大鼠脑创伤后细胞周期蛋白依赖性激酶抑制剂p21（WAF 1/CIP 1）mRNA的表达，以及沿着的调节基因p53和应激反应基因hsp 70 mRNA的表达。伤后3 h，伤侧皮质、白色质、丘脑、CA 2区、部分CA 1、3区及齿状回中p21（WAF 1/CIP 1）mRNA表达明显增加。损伤皮层和海马的杂交信号在伤后24小时恢复到基线水平。另一方面，在整个损伤后时间过程中，在任何脑切片中均未观察到p53 mRNA诱导。伤后3- 6 h，损伤皮质内hsp 70 mRNA有轻度表达，与p21（WAF 1/CIP 1）mRNA表达的暂时性模式相似。这项研究表明，p21（WAF 1/CIP 1）mRNA在创伤性脑损伤后瞬时诱导，不依赖于p53，这可能是一种早期应激反应，通过阻止细胞进入周期并允许DNA修复来保护细胞。NeuroReport 11：2073-2078（C）2000 Lippincott威廉姆斯和威尔金斯。
The expression of the cyclin-dependent kinase inhibitor p21(WAF1/CIP1) mRNA after traumatic brain injury in rats was investigated using an in situ hybridization technique, along with regulating gene p53 and stress response gene hsp70 mRNA levels. At 3h postinjury, p21(WAF1/CIP1) mRNA was markedly increased in the cortex, white matter, thalamus, CA2, a part of CA1,3 and dentate gyrus of the injured side. Hybridization signals remained elevated at bh in injured cortex and hippocampus and returned to the baseline by 24h post-insult. On the other hand, p53 mRNA induction was not observed in any brain sections throughout the post-injury time course. Slight expression of hsp70 mRNA was detected in the injured cortex 3-6h following injury and this was similar to the temporary pattern of p21(WAF1/CIP1) mRNA expression. This study showed p21(WAF1/CIP1) mRNA to be transiently induced after traumatic brain injury, independent of p53, this possibly being an early stress response to protect cells by arresting them in the cycle and allow DNA repair. NeuroReport 11:2073-2078 (C) 2000 Lippincott Williams & Wilkins.