Multiphoton luminescence imaging of chemically functionalized multi-walled carbon nanotubes in cells and solid tumors.
Multiphoton luminescence imaging of chemically functionalized multi-walled carbon nanotubes in cells and solid tumors.
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DOI：
10.1039/c5cc02675j
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发表时间：
2015-05
影响因子：
4.9
通讯作者：
Noelia Rubio;L. Hirvonen;E. Z. Chong;J. Wang;Maxime Bourgognon;H. Kafa;Hatem A.F.M. Hassan;W. Al-Jamal;David McCarthy;Christer Hogstrand;Frederic Festy;K. Al‐Jamal
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文献类型：
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作者：
Noelia Rubio;L. Hirvonen;E. Z. Chong;J. Wang;Maxime Bourgognon;H. Kafa;Hatem A.F.M. Hassan;W. Al-Jamal;David McCarthy;Christer Hogstrand;Frederic Festy;K. Al‐Jamal
报道了化学功能化多壁纳米管（f-MWNTs）的本征非线性光致发光（PL）特性。f-MWNTs在体外固定的肺上皮癌细胞（A549）和Kupffer细胞中成像，并在体内皮下植入的实体瘤中首次使用多光子PL和荧光寿命成像（FLIM）。在近红外激发区域（10750 -950 nm）的多光子成像，在本研究中采用的无标记的方式，提供了最佳的灵敏度和分辨率，以跟踪f-MWNT细胞内的隔间，并促进肿瘤成像和前哨淋巴结跟踪体内。更广泛的应用包括在生物环境中f-MWNTs的实时成像中采用该技术以促进图像引导的药物递送。
The intrinsic nonlinear photoluminescence (PL) property of chemically functionalized multi-walled nanotubes MWNTs (f-MWNTs) is reported in this study. f-MWNTs are imaged in fixed lung epithelial cancer cells (A549) and Kupffer cells in vitro, and in subcutaneously implanted solid tumors in vivo, for the first time, using multiphoton PL and fluorescence lifetime imaging (FLIM). Multiphoton imaging in the near-infrared excitation region (∼750-950 nm), employed in this study in a label-free manner, provides sensitivity and resolution optimal to track f-MWNTs within intra-cellular compartments and facilitates tumour imaging and sentinel lymph node tracking in vivo. Wider applications include employing this technique in live imaging of f-MWNTs in biological milieu to facilitate image-guided drug delivery.