Measurement of the extinction coefficient of prostate specific antigen using interference and absorbance optics in the Optima XL-A analytical ultracentrifuge
Measurement of the extinction coefficient of prostate specific antigen using interference and absorbance optics in the Optima XL-A analytical ultracentrifuge
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在 Optima XL-A 分析超速离心机中使用干涉和吸收光学器件测量前列腺特异性抗原的消光系数
DOI：
10.1007/bfb0114086
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
P. Voelker
中科院分区：
文献类型：
--
作者：
P. Voelker
当生化反应需要精确数量的物质时，或者当将吸光度单位转换为浓度单位时，建立准确的消光系数通常是有意义的。虽然重量法可以提供浓度的快速估计，但盐或杂质的存在会对其准确性产生不利影响。当需要更准确地估计消光系数时，通常使用分析式超离心。该技术已应用于前列腺特异性抗原（PSA），并通过沉淀分析验证了氨基酸分析估计的消光系数值。
Establishment of an accurate extinction coefficient is often of interest when a precise amount of material is required for a biochemical reaction or when converting an association constant from absorbance units into concentration units. Although a gravimetric approach can provide a quick estimate of the concentration, the presence of salts or impurities can adversely affect its accuracy. Analytical ultracentrifugation is often used when more accurate estimates of the extinction coefficient are required. This technique has been applied to prostate specific antigen (PSA), and the value of the extinction coefficient estimated by amino acid analysis is confirmed by sedimentation analysis.